Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken

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dc.contributor.authorHussain, Hafiz Iftikharen
dc.contributor.authorIqbal, Zahiden
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorHuang, Deyuen
dc.contributor.authorSattar, Adeelen
dc.contributor.authorHao, Haihongen
dc.contributor.authorYuan, Zonghuien
dc.date.accessioned2020-09-21T16:10:28Zen
dc.date.available2020-09-21T16:10:28Zen
dc.date.issued2017-08-21en
dc.date.updated2020-09-21T16:10:25Zen
dc.description.abstractNumerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of poultry E. coli strains in vitro and in vivo via antibiotic susceptibility, biofilm formation and adhesion, and invasion and intracellular survivability assays in Caco-2 and Raw 264.7 cell lines as well as the determination of the median lethal dose in two-day old chickens. A clinical pathogenic multidrug-resistant isolate, E. coli 381, isolated from broilers, was found to be highly virulent in cell culture and 1000-fold more virulent in a chicken model than other strains; accordingly, the isolate was subsequently selected for transcriptome analysis. The comparative gene expression profile of MDR E. coli 381 and the reference human strain E. coli ATCC 25922 was completed with Illumina HiSeq. 2500 transcriptome analysis. Differential gene expression analysis indicates that there are multiple pathways involved in the resistance and virulence of this highly virulent strain. The results garnered from this study provide critical information about the highly virulent MDR E. coli strain of poultry origin and warrant further investigation due to its significant threat to public health.en
dc.description.versionPublished versionen
dc.format.extent15 page(s)en
dc.format.mediumElectronicen
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 8335 (Article number)en
dc.identifier.doihttps://doi.org/10.1038/s41598-017-07798-1en
dc.identifier.eissn2045-2322en
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.other10.1038/s41598-017-07798-1 (PII)en
dc.identifier.pmid28827616 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/100019en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMEDIATED QUINOLONE RESISTANCEen
dc.subjectBIOFILM FORMATIONen
dc.subjectANTIBIOTIC-RESISTANCEen
dc.subjectTRANSPORTER GENESen
dc.subjectSTRESS-RESPONSEen
dc.subjectEFFLUXen
dc.subjectSPECTRUMen
dc.subjectANIMALSen
dc.subjectSTRAINSen
dc.subjectIDENTIFICATIONen
dc.subject.meshAnimalsen
dc.subject.meshChickensen
dc.subject.meshEscherichia colien
dc.subject.meshEscherichia coli Infectionsen
dc.subject.meshPoultry Diseasesen
dc.subject.meshBacterial Proteinsen
dc.subject.meshVirulence Factorsen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshBacterial Adhesionen
dc.subject.meshDrug Resistance, Multiple, Bacterialen
dc.subject.meshVirulenceen
dc.subject.meshTranscriptomeen
dc.titleVirulence and transcriptome profile of multidrug-resistant Escherichia coli from chickenen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2017-07-03en
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Techen

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