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The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes

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dc.contributor.authorRyazansky, Sergei S.en
dc.contributor.authorChen, Chujiaen
dc.contributor.authorPotters, Marken
dc.contributor.authorNaumenko, Anastasia N.en
dc.contributor.authorLukyanchikova, Varvaraen
dc.contributor.authorMasri, Reem A.en
dc.contributor.authorBrusentsov, Ilya I.en
dc.contributor.authorKaragodin, Dmitriy A.en
dc.contributor.authorYurchenko, Andrey A.en
dc.contributor.authordos Anjos, Vitor L.en
dc.contributor.authorHaba, Yukien
dc.contributor.authorRose, Noah H.en
dc.contributor.authorHoffman, Jinnaen
dc.contributor.authorGuo, Rongen
dc.contributor.authorMenna, Theresaen
dc.contributor.authorKelley, Melissaen
dc.contributor.authorFerrill, Emilyen
dc.contributor.authorSchultz, Karen E.en
dc.contributor.authorQi, Yuminen
dc.contributor.authorSharma, Atashien
dc.contributor.authorDeschamps, Stéphaneen
dc.contributor.authorLlaca, Victoren
dc.contributor.authorMao, Chunhongen
dc.contributor.authorMurphy, Terence D.en
dc.contributor.authorBaricheva, Elina M.en
dc.contributor.authorEmrich, Scotten
dc.contributor.authorFritz, Megan L.en
dc.contributor.authorBenoit, Joshua B.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorMcBride, Carolyn S.en
dc.contributor.authorTu, Zhijianen
dc.contributor.authorSharakhova, Maria V.en
dc.date.accessioned2024-01-29T18:24:30Zen
dc.date.available2024-01-29T18:24:30Zen
dc.date.issued2024-01-25en
dc.date.updated2024-01-28T04:19:44Zen
dc.description.abstractBackground: Understanding genome organization and evolution is important for species involved in transmission of human diseases, such as mosquitoes. Anophelinae and Culicinae subfamilies of mosquitoes show striking differences in genome sizes, sex chromosome arrangements, behavior, and ability to transmit pathogens. However, the genomic basis of these differences is not fully understood. Methods: In this study, we used a combination of advanced genome technologies such as Oxford Nanopore Technology sequencing, Hi-C scaffolding, Bionano, and cytogenetic mapping to develop an improved chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus. Results: We then used this assembly to annotate odorant receptors, odorant binding proteins, and transposable elements. A genomic region containing male-specific sequences on chromosome 1 and a polymorphic inversion on chromosome 3 were identified in the Cx. quinquefasciatus genome. In addition, the genome of Cx. quinquefasciatus was compared with the genomes of other mosquitoes such as malaria vectors An. coluzzi and An. albimanus, and the vector of arboviruses Ae. aegypti. Our work confirms significant expansion of the two chemosensory gene families in Cx. quinquefasciatus, as well as a significant increase and relocation of the transposable elements in both Cx. quinquefasciatus and Ae. aegypti relative to the Anophelines. Phylogenetic analysis clarifies the divergence time between the mosquito species. Our study provides new insights into chromosomal evolution in mosquitoes and finds that the X chromosome of Anophelinae and the sex-determining chromosome 1 of Culicinae have a significantly higher rate of evolution than autosomes. Conclusion: The improved Cx. quinquefasciatus genome assembly uncovered new details of mosquito genome evolution and has the potential to speed up the development of novel vector control strategies.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Biology. 2024 Jan 25;22(1):16en
dc.identifier.doihttps://doi.org/10.1186/s12915-024-01825-0en
dc.identifier.urihttps://hdl.handle.net/10919/117716en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleThe chromosome-scale genome assembly for the West Nile vector <i>Culex quinquefasciatus</i> uncovers patterns of genome evolution in mosquitoesen
dc.title.serialBMC Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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