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Alphavirus Particles Can Assemble with an Alternate Triangulation Number

dc.contributor.authorKaelber, Jason T.en
dc.contributor.authorChmielewski, Daviden
dc.contributor.authorChiu, Wahen
dc.contributor.authorAuguste, A. Jonathanen
dc.date.accessioned2022-12-12T13:51:48Zen
dc.date.available2022-12-12T13:51:48Zen
dc.date.issued2022-11-27en
dc.date.updated2022-12-09T20:22:53Zen
dc.description.abstractAlphaviruses are spherical, enveloped RNA viruses primarily transmitted by mosquitoes, and cause significant arthritogenic and neurotropic disease in humans and livestock. Previous reports have shown that&mdash;in contrast to prototypical icosahedral viruses&mdash;alphaviruses incorporate frequent defects, and these may serve important functions in the viral life cycle. We confirm the genus-wide pleomorphism in live viral particles and extend our understanding of alphavirus assembly through the discovery of an alternate architecture of Eastern equine encephalitis virus (EEEV) particles. The alternate <i>T</i> = 3 icosahedral architecture differs in triangulation number from the classic <i>T</i> = 4 icosahedral organization that typifies alphaviruses, but the alternate architecture maintains the quasi-equivalence relationship of asymmetric units. The fusion spike glycoproteins are more loosely apposed in the <i>T</i> = 3 form with corresponding changes in the underlying capsid protein lattice. This alternate architecture could potentially be exploited in engineering alphavirus-based particles for delivery of alphaviral or other RNA.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationKaelber, J.T.; Chmielewski, D.; Chiu, W.; Auguste, A.J. Alphavirus Particles Can Assemble with an Alternate Triangulation Number. Viruses 2022, 14, 2650.en
dc.identifier.doihttps://doi.org/10.3390/v14122650en
dc.identifier.urihttp://hdl.handle.net/10919/112840en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectalphavirusen
dc.subjectquasi-equivalenceen
dc.subjecttriangulation numberen
dc.subjectTogaviridaeen
dc.subjectvirus assemblyen
dc.subjectcryo-electron microscopyen
dc.titleAlphavirus Particles Can Assemble with an Alternate Triangulation Numberen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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