Effects of high temperature and disinfectants on the viability of Sarcocystis neurona sporocysts

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dc.contributor.authorDubey, Jitender P.en
dc.contributor.authorSaville, W. J. A.en
dc.contributor.authorSreekumar, C.en
dc.contributor.authorShen, S. K.en
dc.contributor.authorLindsay, David S.en
dc.contributor.authorPena, H. F. J.en
dc.contributor.authorVianna, M. C. B.en
dc.contributor.authorGennari, S. M.en
dc.contributor.authorReed, S. M.en
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:13Zen
dc.date.available2014-06-20T14:13:13Zen
dc.date.issued2002-12en
dc.description.abstractThe effect of moist heat and several disinfectants on Sarcocystis neurona sporocysts was investigated. Sporocysts (4 million) were suspended in water and heated to 50, 55, 60, 65, and 70 C for various times and were then bioassayed in interferon gamma gene knockout (KO) mice. Sporocysts heated to 50 C for 60 min and 55 C for 5 min were infective to KO mice, whereas sporocysts heated to 55 C for 15 min and 60 C or more for I min were rendered noninfective to mice. Treatment with bleach (10, 20, and 100%), 2% chlorhexidine, 1% betadine, 5% o-benzyl-p-chlorophenol, 12.56% phenol, 6% benzyl ammonium chloride, and 10% formalin was not effective in killing sporocysts. Treatment with undiluted ammonium hydroxide (29.5% ammonia) for 1 hr killed sporocysts, but treatment with a 10-fold dilution (2.95% ammonia) for 6 hr did not kill sporocysts. These data indicate that heat treatment is the most effective means of killing S. neurona sporocysts in the horse feed or in the environment.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJ. P. Dubey, W. J. Saville, C. Sreekumar, S. K. Shen, D. S. Lindsay, H. F. Pena, M. C. Vianna, S. M. Gennari, and S. M. Reed (2002). "Effects of High Temperature and Disinfectants on the Viability of Sarcocystis neurona Sporocysts," Journal of Parasitology, Vol. 88, No. 6, pp. 1252-1254. doi: http://dx.doi.org/10.1645/0022-3395(2002)088[1252:EOHTAD]2.0.CO;2en
dc.identifier.doihttps://doi.org/10.1645/0022-3395(2002)088[1252:eohtad]2.0.co;2en
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49041en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/0022-3395%282002%29088%5B1252%3AEOHTAD%5D2.0.CO%3B2en
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectequine protozoal myeloencephalitisen
dc.subjectopossums didelphis-virginianaen
dc.subjectexperimental inductionen
dc.subjectinfectionsen
dc.subjectraccoonsen
dc.subjectmiceen
dc.subjectparasitologyen
dc.titleEffects of high temperature and disinfectants on the viability of Sarcocystis neurona sporocystsen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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