Characterization of a 4.0 kilobase plasmid from Pasteurella multocida

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dc.contributor.authorMcGonagle, Lynnen
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T21:49:50Zen
dc.date.adate2013-11-15en
dc.date.available2014-03-14T21:49:50Zen
dc.date.issued1989-07-05en
dc.date.rdate2013-11-15en
dc.date.sdate2013-11-15en
dc.description.abstractA 4.0 Kb (2.64 Mdal) plasmid was isolated from a fowl cholera strain of Pasteurella multocida (the Larsen strain) by alkaline lysis and cesium chloride purification. The plasmid, designated pLAR-1, was characterized in terms of its size and restriction sites. The restriction patterns produced by fourteen endonucleases were used to generate a restriction map. Five restriction enzymes cleaved the plasmid at multiple sites. Two enzymes, Bgl II and Sal I had unique sites on pLAR-1. Twelve of the fifty six strains of P. multocida surveyed contained plasmids of different sizes which hybridized to pLAR-1. Strains containing homologous plasmids were variable in serotype, dermonecrotoxin production, and origin (both in terms of the host and locale). pLAR-1 did not encode any of the enzymes necessary for the biochemical pathways contained within the API-20E strip or siderophore production. pLAR-1 was cloned into the BamH I site of pBR322. Resultant clones were approximately 8.363 Kb in length, ampicillin resistant and tetracycline sensitive. The pLAR-1en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 62 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11152013-040537en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11152013-040537/en
dc.identifier.urihttp://hdl.handle.net/10919/45762en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1989.M3275.pdfen
dc.relation.isformatofOCLC# 20766127en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1989.M3275en
dc.subject.lcshAvian influenza A virus -- Researchen
dc.titleCharacterization of a 4.0 kilobase plasmid from Pasteurella multocidaen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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