Orb weaver capture thread biomechanics and evolution

Kelly, Sean D.

Orb weaver capture thread biomechanics and evolution

dc.contributor.author	Kelly, Sean D.	en
dc.contributor.committeechair	Opell, Brent D.	en
dc.contributor.committeemember	Uyeda, Josef C.	en
dc.contributor.committeemember	Munoz, Martha	en
dc.contributor.department	Biological Sciences	en
dc.date.accessioned	2021-12-30T07:00:12Z	en
dc.date.available	2021-12-30T07:00:12Z	en
dc.date.issued	2020-07-07	en
dc.description.abstract	Orb weavers intercept insects using non-hardening bioadhesive droplets, supported by two flagelliform fibers. Droplets contain an adhesive glycoprotein core and aqueous layer that confers hygroscopicity. The first study investigates the durability of these droplets to cycling, or repeatedly adhering, extending, and pulling off. Droplets of four species proved resilient, cycling 40 times. Cycling, coupled with droplet humidity responsiveness, qualifies them as smart materials. However, thread adhesion is complex, relying on an integrated performance of multiple droplets and the flagelliform fibers. As insects struggle, the flagelliform fibers bow and the droplets extend, forming a suspension bridge configuration whose biomechanics sum the adhesion of droplets and dissipate the energy of struggling insects. Given this performance, the second study predicts that the material properties of both thread components have evolved in a complementary way. Comparative phylogenetics of 14 study species revealed that their elastic moduli are correlated, with glycoproteins being six times more elastic than flagelliform fibers. Spider mass affects the amount of each material, but not their properties. Since glycoprotein performance changes with humidity, we hypothesized that orb weavers generate greater adhesion at their foraging humidity. After delimiting low and high humidity species groups (eight and six species, respectively), bridge force was determined as total contributing droplet adhesion at three humidities. Only three spiders generated greater adhesion outside of their foraging humidity. The distribution of force along a suspension bridge differed from a previously reported pattern. We also characterize the sheet configuration, which generates force similar to suspension bridges.	en
dc.description.abstractgeneral	In nature, adhesives are used for a variety of functions. Many animals use adhesives use adhesives when climbing. Examples include toe pads of geckos, tarsal pads of ants, and tube feet of and sea urchins. Here, adhesion is repeatedly generated and released as the animal moves. However, some animals depend on permanent adhesives to anchor to surfaces. Marine mussels and barnacles, whose adult forms are sessile, use adhesives to resist the powerful action of waves and currents. Adhesion also plays a critical role in prey capture, where it prevents prey from escaping. The sticky droplets of a sundew plants and the adhesive capture threads of spider orb webs trap flies. Biologists and engineers study these bioadiehsives in search of inspiration and principles that will guide the development of new materials, including adhesives that function underwater, harden rapidly, or remaining pliable after adhering. This study investigated the material properties of capture threads spun by orb weaving spiders, which rely on non-hardening sticky droplets, supported by two protein fibers to capture insects. Inside each droplet is an adhesive core allows droplets to adhere to an insect and to extend as it struggles to escape. Surrounding this core is an aqueous layer that attracts atmospheric water, causing droplets to track changes in ambient humidity. A study of the cycling (or reusability) of four species' droplets repeatedly adhered a droplet to a surface and extending it to pull-off. These droplets were very resilient, cycling 40 times. Cycling, coupled with droplet humidity responsiveness, qualifies them as smart materials. However, prey capture is more complex, relying on the integration of multiple droplets and their supporting flagelliform fibers. As insects struggle, these fibers bow and the droplets extend, forming a suspension bridge configuration whose biomechanics sum the adhesion of droplets to resist an insect escape. The threads of 14 species were examined to test the hypothesis that material properties of both thread components have evolved in a complementary way to optimize adhesive performance. This revealed that the elasticities of the two capture thread components were correlated, with support fiber elasticity being greater. Capture threads generated the greatest adhesion at humidities during times that a spider feeds, although the distribution of this force across a suspension bridge showed different patterns among the species. The functional integration of a capture thread's components and its ability to respond to environmental humidity gives it exciting biomimicry potential.	en
dc.description.degree	Master of Science	en
dc.format.medium	ETD	en
dc.identifier.other	vt_gsexam:26699	en
dc.identifier.uri	http://hdl.handle.net/10919/107285	en
dc.publisher	Virginia Tech	en
dc.rights	In Copyright	en
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/	en
dc.subject	Bioadhesive	en
dc.subject	Biomechanics	en
dc.subject	Flagelliform fibers	en
dc.subject	Glycoprotein	en
dc.subject	Orb weaver	en
dc.title	Orb weaver capture thread biomechanics and evolution	en
dc.type	Thesis	en
thesis.degree.discipline	Biological Sciences	en
thesis.degree.grantor	Virginia Polytechnic Institute and State University	en
thesis.degree.level	masters	en
thesis.degree.name	Master of Science	en