O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis
| dc.contributor.author | Cao, Yu | en |
| dc.contributor.author | Zhang, Meng | en |
| dc.contributor.author | Li, Ye | en |
| dc.contributor.author | Lu, Jingjing | en |
| dc.contributor.author | Zhou, Wanhui | en |
| dc.contributor.author | Li, Xiaoshuang | en |
| dc.contributor.author | Shi, Hao | en |
| dc.contributor.author | Xu, Bin | en |
| dc.contributor.author | Li, Shize | en |
| dc.date.accessioned | 2022-11-29T13:18:00Z | en |
| dc.date.available | 2022-11-29T13:18:00Z | en |
| dc.date.issued | 2022-11-22 | en |
| dc.date.updated | 2022-11-24T14:43:27Z | en |
| dc.description.abstract | Cold stress disturbs cellular metabolic and energy homeostasis, which is one of the causes of stress-induced illnesses. O-GlcNAcylation is a nutrient-sensing pathway involved in a myriad of cellular processes. It plays a key role in metabolic homeostasis. Nevertheless, a specific sensing mechanism linking skeletal muscle to O-GlcNAcylation in cold stress is unknown. In this study, O-GlcNAcylation of SIRT1 was targeted to explore the mechanism of skeletal muscle adaptation to cold stress. <i>Ogt</i> mKO aggravated skeletal muscle fibrosis induced by cold stress. At the same time, <i>Ogt</i> gene deletion accelerated the homeostasis imbalance and oxidative stress of skeletal muscle mitochondria induced by cold stress. In vitro results showed that inhibition of SIRT1’s O-GlcNAcylation accelerated mild hypothermia induced mitochondrial homeostasis in mouse myogenic cells (C2C12 cells). However, overexpression of SIRT1’s O-GlcNAcylation improved the above phenomena. Thus, these results reveal a protective role of OGT-SIRT1 in skeletal muscle’s adaptation to cold stress, and our findings will provide new avenues to combat stress-induced diseases. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Cao, Y.; Zhang, M.; Li, Y.; Lu, J.; Zhou, W.; Li, X.; Shi, H.; Xu, B.; Li, S. O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis. Int. J. Mol. Sci. 2022, 23, 14520. | en |
| dc.identifier.doi | https://doi.org/10.3390/ijms232314520 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/112724 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | cold stress | en |
| dc.subject | skeletal muscle | en |
| dc.subject | metabolic homeostasis imbalance | en |
| dc.subject | SIRT1 | en |
| dc.subject | O-GlcNAcylation | en |
| dc.title | O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis | en |
| dc.title.serial | International Journal of Molecular Science | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |