Characterizing the transport and utilization of the neurotransmitter GABA in the bacterial pathogen Brucella abortus

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dc.contributor.authorBudnick, James A.en
dc.contributor.authorSheehan, Lauren M.en
dc.contributor.authorBenton, Angela H.en
dc.contributor.authorPitzer, Joshua E.en
dc.contributor.authorKang, Linen
dc.contributor.authorMichalak, Pawelen
dc.contributor.authorRoop, R. Martin IIen
dc.contributor.authorCaswell, Clayton C.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-09-29T16:39:57Zen
dc.date.available2021-09-29T16:39:57Zen
dc.date.issued2020-08-26en
dc.date.updated2021-09-29T16:39:54Zen
dc.description.abstractThe neurotransmitter gamma-aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the human brain; however, it is becoming more evident that this non-proteinogenic amino acid plays multiple physiological roles in biology. In the present study, the transport and function of GABA is studied in the highly infectious intracellular bacterium Brucella abortus. The data show that 3H-GABA is imported by B. abortus under nutrient limiting conditions and that the small RNAs AbcR1 and AbcR2 negatively regulate this transport. A specific transport system, gts, is responsible for the transport of GABA as determined by measuring 3H-GABA transport in isogenic deletion strains of known AbcR1/2 regulatory targets; however, this locus is unnecessary for Brucella infection in BALB/c mice. Similar assays revealed that 3H-GABA transport is uninhibited by the 20 standard proteinogenic amino acids, representing preference for the transport of 3H-GABA. Metabolic studies did not show any potential metabolic utilization of GABA by B. abortus as a carbon or nitrogen source, and RNA sequencing analysis revealed limited transcriptional differences between B. abortus 2308 with or without exposure to GABA. While this study provides evidence for GABA transport by B. abortus, questions remain as to why and when this transport is utilized during Brucella pathogenesis.en
dc.description.versionPublished versionen
dc.format.extent19 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e0237371 (Article number)en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0237371en
dc.identifier.eissn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.issue8en
dc.identifier.orcidCaswell, Clayton [0000-0003-3793-8804]en
dc.identifier.otherPONE-D-20-12100 (PII)en
dc.identifier.pmid32845904en
dc.identifier.urihttp://hdl.handle.net/10919/105109en
dc.identifier.volume15en
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000566205500041&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectGLUTAMATE-DECARBOXYLASEen
dc.subjectPLANT GABAen
dc.subjectACIDen
dc.subjectMETABOLISMen
dc.subjectRESISTANCEen
dc.subjectVIRULENCEen
dc.subjectGROWTHen
dc.subjectGENESen
dc.subject.meshAnimalsen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshMiceen
dc.subject.meshBrucella abortusen
dc.subject.meshgamma-Aminobutyric Aciden
dc.subject.meshGlutamic Aciden
dc.subject.meshNeurotransmitter Agentsen
dc.subject.meshBiological Transporten
dc.titleCharacterizing the transport and utilization of the neurotransmitter GABA in the bacterial pathogen Brucella abortusen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2020-08-04en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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