Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato

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dc.contributor.authorLaimbeer, F. Parker E.en
dc.contributor.authorBargmann, Bastiaan O. R.en
dc.contributor.authorHolt, Sarah H.en
dc.contributor.authorPratt, Trentonen
dc.contributor.authorPeterson, Brenda A.en
dc.contributor.authorDoulis, Andreas G.en
dc.contributor.authorBuell, C. Robinen
dc.contributor.authorVeilleux, Richard E.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-03-02T19:02:01Zen
dc.date.available2021-03-02T19:02:01Zen
dc.date.issued2020-08-27en
dc.description.abstractAnthocyanins are pigmented secondary metabolites produced via the flavonoid biosynthetic pathway and play important roles in plant stress responses, pollinator attraction, and consumer preference. Using RNA-sequencing analysis of a cross between diploid potato (Solanum tuberosum L.) lines segregating for flower color, we identified a homolog of the ANTHOCYANIN 2 (AN2) gene family that encodes a MYB transcription factor, herein termed StFlAN2, as the regulator of anthocyanin production in potato corollas. Transgenic introduction of StFlAN2 in white-flowered homozygous doubled-monoploid plants resulted in a recovery of purple flowers. RNA-sequencing revealed the specific anthocyanin biosynthetic genes activated by StFlAN2 as well as expression differences in genes within pathways involved in fruit ripening, senescence, and primary metabolism. Closer examination of the locus using genomic sequence analysis revealed a duplication in the StFlAN2 locus closely associated with gene expression that is likely attributable to nearby genetic elements. Taken together, this research provides insight into the regulation of anthocyanin biosynthesis in potato while also highlighting how the dynamic nature of the StFlAN2 locus may affect expression.en
dc.description.sponsorshipThis research was supported in part by National Science Foundation Award number IOS-1237969, “Unraveling the Heterozygosity, Allelic Composition, and Copy Number Variation of Potato” to CRB and RV and USDA Special Grant 2014-34141-22266 (University of Maine) to RV. USDA Hatch Project VA-135853, the Department of Horticulture, Virginia Tech and the Translational Plant Science Program, Virginia Tech.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1534/g3.120.401684en
dc.identifier.urihttp://hdl.handle.net/10919/102523en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherGenetics Society of Americaen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSolanumen
dc.subjecttuberosumen
dc.subjectbulk segreganten
dc.subjectanalysisen
dc.subjectcopy numberen
dc.subjectvariationen
dc.subjecttransgenic complementationen
dc.subjectmonoploiden
dc.titleCharacterization of the F Locus Responsible for Floral Anthocyanin Production in Potatoen
dc.title.serialG3: Genes|Genomes|Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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