Separation and Detection of 2,3-Dihydroxybenzoic Acid

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dc.contributor.authorHooper, Stephanie Elaineen
dc.contributor.committeechairAnderson, Mark R.en
dc.contributor.committeememberCrawford, T. Danielen
dc.contributor.committeememberLong, Gary L.en
dc.contributor.departmentChemistryen
dc.date.accessioned2011-08-06T16:06:22Zen
dc.date.adate2004-08-26en
dc.date.available2011-08-06T16:06:22Zen
dc.date.issued2002-04-17en
dc.date.rdate2010-10-07en
dc.date.sdate2004-08-24en
dc.description.abstractIn Parkinson's disease, severe damage to nigrostriatal neurons causes a depletion of the neurotransmitter dopamine (DA). Oxidative stress on the brain is thought to contribute to neuron cell death and to the onset of Parkinson's disease. Reactive oxygen radicals produced during oxidative stress have been implicated as an initiator of neuron destruction. Glutamate, an excitatory neurotransmitter, can initiate OH radical formation when present in excess. Oxidative stress on the brain caused by glutamate overflow may be monitored by trapping the OH radicals with salicylic acid to produce 2,3-dihydroxybenzoic acid (2,3-DHBA). Determination of this product is initially performed using capillary zone electrophoresis (CZE) coupled with UV detection to establish optimum separation conditions. These conditions were applied for rapid, efficient, and sensitive determination of 2,3-DHBA by CZE coupled with electrochemical detection. Quick and sensitive detection of 2,3-DHBA is essential in monitoring OH radical generation and identifying its role in Parkinson's disease.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.otheretd-08242004-154958en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08242004-154958en
dc.identifier.urihttp://hdl.handle.net/10919/10112en
dc.publisherVirginia Techen
dc.relation.haspartSHThesis2.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectelectrochemical and UV detectionen
dc.subjectcapillary electrophoresisen
dc.subjectoxidative stressen
dc.titleSeparation and Detection of 2,3-Dihydroxybenzoic Aciden
dc.typeThesisen
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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