Impact of extrinsic incubation temperature on natural selection during Zika virus infection of Aedes aegypti and Aedes albopictus

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dc.contributor.authorMurrieta, Reyes A.en
dc.contributor.authorGarcia-Luna, Selene M.en
dc.contributor.authorMurrieta, Deedra J.en
dc.contributor.authorHalladay, Garethen
dc.contributor.authorYoung, Michael C.en
dc.contributor.authorFauver, Joseph R.en
dc.contributor.authorGendernalik, Alexen
dc.contributor.authorWeger-Lucarelli, Jamesen
dc.contributor.authorRückert, Claudiaen
dc.contributor.authorEbel, Gregory D.en
dc.date.accessioned2022-01-12T13:34:29Zen
dc.date.available2022-01-12T13:34:29Zen
dc.date.issued2021-11-09en
dc.date.updated2022-01-12T13:34:25Zen
dc.description.abstractArthropod-borne viruses (arboviruses) require replication across a wide range of temperatures to perpetuate. While vertebrate hosts tend to maintain temperatures of approximately 37°C-40°C, arthropods are subject to ambient temperatures which can have a daily fluctuation of > 10°C. Temperatures impact vector competence, extrinsic incubation period, and mosquito survival unimodally, with optimal conditions occurring at some intermediate temperature. In addition, the mean and range of daily temperature fluctuations influence arbovirus perpetuation and vector competence. The impact of temperature on arbovirus genetic diversity during systemic mosquito infection, however, is poorly understood. Therefore, we determined how constant extrinsic incubation temperatures of 25°C, 28°C, 32°C, and 35°C control Zika virus (ZIKV) vector competence and population dynamics within Aedes aegypti and Aedes albopictus mosquitoes. We also examined fluctuating temperatures which better mimic field conditions in the tropics. We found that vector competence varied in a unimodal manner for constant temperatures peaking between 28°C and 32°C for both Aedes species. Transmission peaked at 10 days post-infection for Aedes aegypti and 14 days for Aedes albopictus. Conversely, fluctuating temperature decreased vector competence. Using RNA-seq to characterize ZIKV population structure, we identified that temperature alters the selective environment in unexpected ways. During mosquito infection, constant temperatures more often elicited positive selection whereas fluctuating temperatures led to strong purifying selection in both Aedes species. These findings demonstrate that temperature has multiple impacts on ZIKV biology, including major effects on the selective environment within mosquitoes.en
dc.description.versionPublished versionen
dc.format.extentPages e1009433en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1009433en
dc.identifier.eissn1553-7374en
dc.identifier.issn1553-7366en
dc.identifier.issue11en
dc.identifier.orcidWeger, James [0000-0002-9483-1694]en
dc.identifier.otherPMC8629396en
dc.identifier.otherPPATHOGENS-D-21-00423 (PII)en
dc.identifier.pmid34752502en
dc.identifier.urihttp://hdl.handle.net/10919/107561en
dc.identifier.volume17en
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/34752502en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectVirologyen
dc.subject0605 Microbiologyen
dc.subject1107 Immunologyen
dc.subject1108 Medical Microbiologyen
dc.subject.meshAedesen
dc.subject.meshAnimalsen
dc.subject.meshChlorocebus aethiopsen
dc.subject.meshGenetic Fitnessen
dc.subject.meshMosquito Vectorsen
dc.subject.meshSalivaen
dc.subject.meshSelection, Geneticen
dc.subject.meshTemperatureen
dc.subject.meshVero Cellsen
dc.subject.meshViral Loaden
dc.subject.meshZika Virusen
dc.subject.meshZika Virus Infectionen
dc.titleImpact of extrinsic incubation temperature on natural selection during Zika virus infection of Aedes aegypti and Aedes albopictusen
dc.title.serialPLoS Pathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
dc.type.otherResearch Support, N.I.H., Extramuralen
dcterms.dateAccepted2021-10-18en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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