Sensitive cross section of a bacterium, Escherichia coli, to fast neutrons

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dc.contributor.authorKeefe, William Edwarden
dc.contributor.committeechairRobeson, Andrewen
dc.contributor.committeememberMoore, Walter E. C.en
dc.contributor.departmentPhysicsen
dc.date.accessioned2014-03-14T21:49:17Zen
dc.date.adate2012-11-09en
dc.date.available2014-03-14T21:49:17Zen
dc.date.issued1964-06-05en
dc.date.rdate2012-11-09en
dc.date.sdate2012-11-09en
dc.description.abstractAn experiment was performed to determine the sensitive area of a lyophilized bacterium, <u>Escherichia coli</u>, to fast (14Mev.) neutrons. The neutrons were obtained from the H³(d,n)He⁴ reaction produced in the Cockeroft-Walton accelerator at Virginia Polytechnic Institute. The reaction provides a neutron source which is almost monoenergetic, symmetrical, has a low level of associated gamma rays, and gives a large number of neutrons per unit time. To insure that the maximum number of neutrons per cm² per unit time pass through the samples, a special target holder, and sample holder were constructed to position the samples close to the target's center. Source strength was determined with a long counter. The flux at the site of irradiation was determined by numerical integration. <p>One half of the lyophilized culture tubes were held for controls, the others were irradiated. after the letter had been irradiated, all were opened aseptically, plated on agar, and colonies counted. Because of the extreme variation between colony counts of lyophilized samples, a weighted least squares analysis was performed to estimate the sensitive area. The total flux to which the bacteria were subjected was approximately 10<sup>12</sup> neutrons/cm<sup>2</sup> and the sensitive area was estimated to be lO<sup>-l2</sup> cm<sup>2</sup>. This agrees with values obtained by others for this bacterium irradiated in aqueous solution and on agar plates. Since the average size of <u>Escherichia coli</u> is .5 x 1.5 microns, the sensitive area is approximately 0.01% or the total cell area.en
dc.description.degreeMaster of Scienceen
dc.format.extent53 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11092012-040128en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11092012-040128/en
dc.identifier.urihttp://hdl.handle.net/10919/45558en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1964.K428.pdfen
dc.relation.isformatofOCLC# 21379752en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1964.K428en
dc.subject.lcshEscherichia colien
dc.subject.lcshNeutrons -- Physiological effecten
dc.titleSensitive cross section of a bacterium, Escherichia coli, to fast neutronsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplinePhysicsen
thesis.degree.grantorVirginia Polytechnic Instituteen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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