Pseudomonas aeruginosa Initiates a Rapid and Specific Transcriptional Response during Surface Attachment

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dc.contributor.authorJones, Christopher J.en
dc.contributor.authorGrotewold, Nikolasen
dc.contributor.authorWozniak, Daniel J.en
dc.contributor.authorGloag, Erin S.en
dc.contributor.editorO’Toole, Georgeen
dc.date.accessioned2022-12-14T20:23:01Zen
dc.date.available2022-12-14T20:23:01Zen
dc.date.issued2022-05en
dc.date.updated2022-12-14T19:07:22Zen
dc.description.abstractChronic biofilm infections by Pseudomonas aeruginosa are a major contributor to the morbidity and mortality of patients. The formation of multicellular bacterial aggregates, called biofilms, is associated with increased resistance to antimicrobials and immune clearance and the persistence of infections. Biofilm formation is dependent on bacterial cell attachment to surfaces, and therefore, attachment plays a key role in chronic infections. We hypothesized that bacteria sense various surfaces and initiate a rapid, specific response to increase adhesion and establish biofilms. RNA sequencing (RNA-Seq) analysis identified transcriptional changes of adherent cells during initial attachment, identifying the bacterial response to an abiotic surface over a 1-h period. Subsequent screens investigating the most highly regulated genes in surface attachment identified 4 genes, <i>pfpI</i>, <i>phnA</i>, <i>leuD</i>, and <i>moaE</i>, all of which have roles in both metabolism and biofilm formation. In addition, the transcriptional responses to several different medically relevant abiotic surfaces were compared after initial attachment. Surprisingly, there was a specific transcriptional response to each surface, with very few genes being regulated in response to surfaces in general. We identified a set of 20 genes that were differentially expressed across all three surfaces, many of which have metabolic functions, including molybdopterin cofactor biosynthesis and nitrogen metabolism. This study has advanced the understanding of the kinetics and specificity of bacterial transcriptional responses to surfaces and suggests that metabolic cues are important signals during the transition from a planktonic to a biofilm lifestyle. IMPORTANCE Bacterial biofilms are a significant concern in many aspects of life, including chronic infections of airways, wounds, and indwelling medical devices; biofouling of industrial surfaces relevant for food production and marine surfaces; and nosocomial infections. The effects of understanding surface adhesion could impact many areas of life. This study utilized emerging technology in a novel approach to address a key step in bacterial biofilm development. These findings have elucidated both conserved and surface-specific responses to several disease-relevant abiotic surfaces. Future work will expand on this report to identify mechanisms of biofilm initiation with the aim of identifying bacterial factors that could be targeted to prevent biofilms.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1128/jb.00086-22en
dc.identifier.eissn1098-5530en
dc.identifier.issn0021-9193en
dc.identifier.issue5en
dc.identifier.orcidGloag, Erin [0000-0001-8895-3444]en
dc.identifier.otherPMC9112911en
dc.identifier.pmid35467391en
dc.identifier.urihttp://hdl.handle.net/10919/112889en
dc.identifier.volume204en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/35467391en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPseudomonas aeruginosaen
dc.subjectRNA-Seqen
dc.subjectBiofilmen
dc.subjectSurface adhesionen
dc.subjectSurface attachmenten
dc.subjectEmerging Infectious Diseasesen
dc.subjectInfectious Diseasesen
dc.subjectCystic Fibrosisen
dc.subjectGeneticsen
dc.subjectRare Diseasesen
dc.subjectLungen
dc.subject2.2 Factors relating to the physical environmenten
dc.subject1.1 Normal biological development and functioningen
dc.subject1 Underpinning researchen
dc.subject2 Aetiologyen
dc.subject2.1 Biological and endogenous factorsen
dc.subjectInfectionen
dc.subject.meshHumansen
dc.subject.meshBiofilmsen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshBacterial Adhesionen
dc.title<i>Pseudomonas aeruginosa</i> Initiates a Rapid and Specific Transcriptional Response during Surface Attachmenten
dc.title.serialJournal of Bacteriologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherResearch Support, Non-U.S. Gov'ten
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
dc.type.otherResearch Support, N.I.H., Extramuralen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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