VTechWorks staff will be away for the Thanksgiving holiday beginning at noon on Wednesday, November 27, through Friday, November 29. We will resume normal operations on Monday, December 2. Thank you for your patience.
 

Evaluation of a Monoclonal-based EIA for the Detection of Giardia lamblia and the Identification of the Antigen

Simple item page
dc.contributor.authorBoone, James Hunter M.S.en
dc.contributor.committeechairFalkinham, Joseph O. IIIen
dc.contributor.committeememberKrieg, Noel R.en
dc.contributor.committeememberWilkins, Tracy D.en
dc.contributor.departmentBiologyen
dc.date.accessioned2014-03-14T20:51:27Zen
dc.date.adate1998-05-18en
dc.date.available2014-03-14T20:51:27Zen
dc.date.issued1998-05-05en
dc.date.rdate2012-06-08en
dc.date.sdate1998-05-05en
dc.description.abstractI. A number of commercial enzyme immunoassay (EIA) tests are available for the diagnosis of giardiasis. In a time of rising health-care costs, there is a need for diagnostic tests that are rapid, specific, sensitive and inexpensive. In the first phase of this study, I developed a monoclonal-based EIA, the GIARDIA TEST, with these qualities in mind. This assay's performance characteristics were determined by a comparison study using conventional ova and parasite examination, immunofluorescence antibody test (IFA) and other commercial EIA tests. Studies were done in-house at TechLab, Inc. and at various U.S. medical facilities. Results were statistically analyzed to determine sensitivity (ability of the assay to detect a positive result), specificity (amount of cross-reactivity), predictive positive value (the confidence in a positive result), predictive negative value (the confidence in a negative result) and overall correlation with the reference assay. II. There remain many questions to be answered about the various antigens produced by Giardia lamblia and how they can be utilized as diagnostic markers. In the second phase of this study, I identified and partially characterized the antigen (Ct7 Ag) that reacts with the Ct7 monoclonal antibody (MAb). This MAb is an IgM class mouse immunoglobin that is utilized by the GIARDIA TEST and by an immunofluorence antibody test (IFA) which detects Giardia cysts in water and feces. The results of this study will provide physicians and researchers with detailed information about the Ct7 Ag and why it is a useful marker for giardiasis.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-41598-161051en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-41598-161051/en
dc.identifier.urihttp://hdl.handle.net/10919/36676en
dc.publisherVirginia Techen
dc.relation.haspartTITLE.PDFen
dc.relation.haspartBODY.PDFen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAntigenen
dc.subjectImmunoassayen
dc.subjectGiardiaen
dc.titleEvaluation of a Monoclonal-based EIA for the Detection of Giardia lamblia and the Identification of the Antigenen
dc.typeThesisen
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
TITLE.PDF
Size:
13.56 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
BODY.PDF
Size:
855.35 KB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses