Preparation of Heparin Surface for Quantification of Fibroblast Growth Factor-2 (FGF-2) Binding Using Surface Plasmon Resonance (SPR)

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dc.contributor.authorKirtland, David Randen
dc.contributor.committeechairForsten-Williams, Kimberlyen
dc.contributor.committeememberDavis, Richey M.en
dc.contributor.committeememberDessy, Raymond E.en
dc.contributor.departmentBiomedical Engineering and Sciencesen
dc.date.accessioned2014-03-14T20:38:41Zen
dc.date.adate2005-06-17en
dc.date.available2014-03-14T20:38:41Zen
dc.date.issued2005-05-04en
dc.date.rdate2006-06-17en
dc.date.sdate2005-05-26en
dc.description.abstractA mixed self assembling monolayer (mSAM) chip with attached heparin was developed to analyze heparin-protein interactions using a Reichert Inc, SR7000, surface plasmon resonance (SPR) instrument. The heparin was attached via streptavidin-biotin linkage where the streptavidin was covalently coupled to the mSAM and biotinylated heparin bound to it. These chips were then used to quantify the interactions of fibroblast growth factor-2 (FGF-2) with the surface bound heparin. Kinetic rate constants of association and disassociation were calculated. The association data of FGF-2 with heparin was fit to a single compartment, well-mixed model as the data did not exhibit mass transfer limitations. The results suggested that rebinding was prevalent and observed disassociation rates differed significantly in the presence of competing soluble heparin during disassociation. Our results indicate that the Reichert instrument and mSAM chips can be used to analyze heparin-protein interactions but that a careful protocol, outlined in this thesis, should be followed to obtain optimal data.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05262005-134908en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05262005-134908/en
dc.identifier.urihttp://hdl.handle.net/10919/33265en
dc.publisherVirginia Techen
dc.relation.haspartDRK-Thesis-V-1.1.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectFibroblast Growth Factor-2 (FGF-2) basic Fibroblasen
dc.subjectMixed Self-Assembled Monolayeren
dc.subjectMixed Self Assembling Monolayer (mSAM)en
dc.subjectSurface Plasmon Resonance (SPR)en
dc.subjectGrowth Factoren
dc.subjectBiosensoren
dc.subjectHeparinen
dc.titlePreparation of Heparin Surface for Quantification of Fibroblast Growth Factor-2 (FGF-2) Binding Using Surface Plasmon Resonance (SPR)en
dc.typeThesisen
thesis.degree.disciplineBiomedical Engineering and Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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