VTechWorks staff will be away for the winter holidays starting Tuesday, December 24, 2024, through Wednesday, January 1, 2025, and will not be replying to requests during this time. Thank you for your patience, and happy holidays!
 

Biologically relevant small variations of intra-cellular pH can have significant effect on stability of protein-DNA complexes, including the nucleosome

Simple item page
dc.contributor.authorOnufriev, Alexey V.en
dc.date.accessioned2023-08-22T13:59:00Zen
dc.date.available2023-08-22T13:59:00Zen
dc.date.issued2023-04en
dc.description.abstractStability of a protein-ligand complex may be sensitive to pH of its environment. Here we explore, computationally, stability of a set of protein-nucleic acid complexes using fundamental thermodynamic linkage relationship. The nucleosome, as well as an essentially random selection of 20 protein complexes with DNA or RNA, are included in the analysis. An increase in intracellular/intra-nuclear pH destabilizes most complexes, including the nucleosome. We propose to quantify the effect by Delta Delta G(0.3)- the change in the binding free energy due to pH increase of 0.3 units, corresponding to doubling of the H+ activity; variations of pH of this amplitude can occur in living cells, including in the course of the cell cycle, and in cancer cells relative to normal ones. We suggest, based on relevant experimental findings, a threshold of biological significance of 12 k(B)T (similar to 0.3 kcal/mol) for changes of stability of chromatin-related protein-DNA complexes: a change in the binding affinity above the threshold may have biological consequences. We find that for 70% of the examined complexes,Delta Delta G(0.3) > 12 k(B)T (for 10%,Delta Delta G(0.3) is between 3 and 4 k(B)T). Thus, small but relevant variations of intra-nuclear pH of 0.3 may have biological consequences for many protein-nucleic acid complexes. The binding affinity between the histone octamer and its DNA, which directly affects the DNA accessibility in the nucleosome, is predicted to be highly sensitive to intranuclear pH. A variation of 0.3 units results in Delta Delta G(0.3) similar to 10k(B)T (similar to 6 kcal/mol); for spontaneous unwrapping of 20 bp long entry/exit fragments of the nucleosomal DNA,Delta Delta G(0.3) = 2.2k(B)T; partial disassembly of the nucleosome into the tetrasome is characterized by Delta Delta G(0.3) = 5.2k(B)T. The predicted pH -induced modulations of the nucleosome stability are significant enough to suggest that they may have consequences relevant to the biological function of the nucleosome. Accessibility of the nucleosomal DNA is predicted to positively correlate with pH variations during the cell cycle; an increase in intra-cellular pH seen in cancer cells is predicted to lead to a more accessible nucleosomal DNA; a drop in pH associated with apoptosis is predicted to make nucleosomal DNA less accessible. We speculate that processes that depend on accessibility to the DNA in the nucleosomes, such as transcription or DNA replication, might become upregulated due to relatively small, but nevertheless realistic increases of intranuclear pH.en
dc.description.notesThis work was supported in part by the National Institutes of Health [R01 GM144596].en
dc.description.sponsorshipNational Institutes of Health [R01 GM144596]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmolb.2023.1067787en
dc.identifier.eissn2296-889Xen
dc.identifier.other1067787en
dc.identifier.pmid37143824en
dc.identifier.urihttp://hdl.handle.net/10919/116077en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectprotein DNA complexen
dc.subjectstabilityen
dc.subjectbinding affinityen
dc.subjectpHen
dc.subjectnucleosomeen
dc.subjectDNA accessibilityen
dc.subjecttranscriptionen
dc.titleBiologically relevant small variations of intra-cellular pH can have significant effect on stability of protein-DNA complexes, including the nucleosomeen
dc.title.serialFrontiers in Molecular Biosciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fmolb-10-1067787.pdf
Size:
1.16 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Scholarly Works, Physics
Scholarly Works, Center for Soft Matter and Biological Physics
Scholarly Works, Computer Science