The transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputs

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dc.contributor.authorVan Eck, Leonen
dc.contributor.authorDavidson, Rebecca M.en
dc.contributor.authorWu, Shuchien
dc.contributor.authorZhao, Bingyu Y.en
dc.contributor.authorBotha, Anna-Mariaen
dc.contributor.authorLeach, Jan E.en
dc.contributor.authorLapitan, Nora L. V.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-01-22T04:48:02Zen
dc.date.available2020-01-22T04:48:02Zen
dc.date.issued2014-01-01en
dc.date.updated2020-01-22T04:47:58Zen
dc.description.abstractThe transcription factor WRKY53 is expressed during biotic and abiotic stress responses in cereals, but little is currently known about its regulation, structure and downstream targets. We sequenced the wheat ortholog TaWRKY53 and its promoter region, which revealed extensive similarity in gene architecture and cis-acting regulatory elements to the rice ortholog OsWRKY53, including the presence of stress-responsive abscisic acid-responsive elements (ABRE) motifs and GCC-boxes. Four proteins interacted with the WRKY53 promoter in yeast one-hybrid assays, suggesting that this gene can receive inputs from diverse stress-related pathways such as calcium signalling and senescence, and environmental cues such as drought and ultraviolet radiation. The Ser/Thr receptor kinase ORK10/LRK10 and the apoplastic peroxidase POC1 are two downstream targets for regulation by the WRKY53 transcription factor, predicted based on the presence of W-box motifs in their promoters and coregulation with WRKY53, and verified by electrophoretic mobility shift assay (EMSA). Both ORK10/LRK10 and POC1 are upregulated during cereal responses to pathogens and aphids and important components of the oxidative burst during the hypersensitive response. Taken with our yeast two-hybrid assay which identified a strong protein-protein interaction between microsomal glutathione S-transferase 3 and WRKY53, this implies that the WRKY53 transcriptional network regulates oxidative responses to a wide array of stresses. © 2014 The Author(s).en
dc.description.versionPublished (Publication status)en
dc.format.extentPages 351-362en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s10142-014-0374-3en
dc.identifier.eissn1438-7948en
dc.identifier.issn1438-793Xen
dc.identifier.issue2en
dc.identifier.pmid24777609en
dc.identifier.urihttp://hdl.handle.net/10919/96535en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/24777609en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject0604 Geneticsen
dc.subject0607 Plant Biologyen
dc.subjectPlant Biology & Botanyen
dc.subject.meshOryza sativaen
dc.subject.meshTriticumen
dc.subject.meshAbscisic Aciden
dc.subject.meshPlant Proteinsen
dc.subject.meshTranscription Factorsen
dc.subject.meshUltraviolet Raysen
dc.subject.meshSignal Transductionen
dc.subject.meshTranscription, Geneticen
dc.subject.meshGene Expression Regulation, Planten
dc.subject.meshProtein Bindingen
dc.subject.meshSequence Homology, Nucleic Aciden
dc.subject.meshOxidative Stressen
dc.subject.meshGene Regulatory Networksen
dc.subject.meshProtein Interaction Domains and Motifsen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshPlant Immunityen
dc.subject.meshOryzaen
dc.titleThe transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputsen
dc.title.serialFunctional and Integrative Genomicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2014-04-16en
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen

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