Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
| dc.contributor.author | Gonzalez, Juan H. | en |
| dc.contributor.author | Taylor, Joseph S. | en |
| dc.contributor.author | Reed, Kelsey M. | en |
| dc.contributor.author | Wright, R. Clay | en |
| dc.contributor.author | Bargmann, Bastiaan O. R. | en |
| dc.date.accessioned | 2021-11-11T19:19:25Z | en |
| dc.date.available | 2021-11-11T19:19:25Z | en |
| dc.date.issued | 2021-10-23 | en |
| dc.date.updated | 2021-11-11T14:57:19Z | en |
| dc.description.abstract | Background: Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle. Methods: Conditional overexpression of <i>WUS</i> and <i>ARF5</i>Δ was used to study the effect of timing the overexpression of these morphogenic factors during shoot regeneration from root explants in Arabidopsis. In addition, their effect on auxin-signaling activation was examined by visualization and cytometric quantification of the DR5:GFP auxin-signaling reporter in roots and protoplasts, respectively. Results: The induced expression of both <i>WUS</i> and <i>ARF5</i>Δ led to an activation of auxin signaling in roots. Activation of auxin signaling by <i>WUS</i> and <i>ARF5</i>Δ was further quantified by transient transformation of protoplasts. Ectopic overexpression of both <i>WUS</i> and <i>ARF5</i>Δ enhanced regeneration efficiency, but only during the shoot-induction stage of regeneration and not during the callus-induction stage. Conclusions: The overexpression of <i>WUS</i> and <i>ARF5</i>Δ both lead to activation of auxin signaling. Expression during the shoot-induction stage is critical for the enhancement of shoot regeneration by <i>WUS</i> and <i>ARF5</i>Δ. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Gonzalez, J.H.; Taylor, J.S.; Reed, K.M.; Wright, R.C.; Bargmann, B.O.R. Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration. Plants 2021, 10, 2271. | en |
| dc.identifier.doi | https://doi.org/10.3390/plants10112271 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/106600 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | plant regeneration | en |
| dc.subject | morphogenic factors | en |
| dc.subject | conditional expression | en |
| dc.subject | auxin signaling | en |
| dc.title | Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration | en |
| dc.title.serial | Plants | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |