High-resolution Optical Scanning Holography

Abstract

Optical scanning holography, which was proposed by Poon[1], is a fascinating technology to record holographic information. The technique is applied in the operation of scanning holographic microscopy to record the entire three-dimensional volume of a biological specimen in the form of a hologram. With the data captured, a digital reconstruction or decoding is used to reconstruct the hologram of that such specimen. An accurate reconstruction of the recorded data provides with an in-depth analysis in the area where random noise and other imperfection effects may occur. In this thesis, three different approaches of reconstruction process are presented to provide in high-resolution a comparison between theoretical and experimental reconstruction a hologram of fluorescent beads. The first approach is to use only the experimental pinhole hologram recorded to correlate with the hologram of the object to give the reconstruction of the section. The second approach is to use the propagated pinhole hologram to reconstruct at an arbitrary depth. Finally, the third approach is to reconstruct without using the experimental pinhole hologram but with diffraction theory. Comparing these results in high-resolution gives us analysis of the reconstruction noise due to optical aberration.