Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study
| dc.contributor.author | Yang, Che-Hsueh | en |
| dc.contributor.author | Tung, Min-Che | en |
| dc.contributor.author | Lin, Yi-Sheng | en |
| dc.contributor.author | Hsu, Chao-Yu | en |
| dc.contributor.author | Akhrymuk, Ivan | en |
| dc.contributor.author | Tan, Kok-Tong | en |
| dc.contributor.author | Ou, Yen-Chuan | en |
| dc.contributor.author | Lin, Chi-Chien | en |
| dc.date.accessioned | 2022-10-26T19:28:07Z | en |
| dc.date.available | 2022-10-26T19:28:07Z | en |
| dc.date.issued | 2022-10-25 | en |
| dc.date.updated | 2022-10-26T11:07:54Z | en |
| dc.description.abstract | <b> </b>The study aimed to investigate the therapeutic impact of intravesical instillation of dehydrated human amnion-chorion membrane (HACM) extracts based on the primary pathological feature of interstitial cystitis (IC). We divided 15 female Sprague-Dawley rats into three groups: sham control, IC, and treatment group. IC was induced by 400-µL lipopolysaccharide (1 µg/µL), and it was replaced with normal saline in the sham control group. After IC induction, 300 µL dehydrated HACM extracts (3 mg/kg) were instilled into rats’ urinary bladder weekly for 3 weeks. General histology, inflammatory cytokines, NF-κB, oxidative markers, and western blots results were examined. The urothelial denudation, mast-cell infiltration, and tissues fibrosis were all ameliorated. The elevated TNF-α, IL-1β, IL-6, IL-8, and NF-κB were all down-regulated by dehydrated HACM extracts (<i>p</i> < 0.05). For reactive oxygen species, increased malondialdehyde, decreased superoxide dismutase, and decreased glutathione peroxidase were all reversed (<i>p</i> < 0.05). In apoptosis of IC, elevated Bax and suppressed Bcl-2 were improved (<i>p</i> < 0.05) after instillation. In fibrosis, dysregulated TGFβ/R-Smads/Snail was corrected by the instillation of dehydrated HACM (<i>p</i> < 0.05). In conclusion, dehydrated HACM extracts could be a powerful remedy in treating IC by reconstructing the damaged urothelium, reducing mast-cell infiltration and inflammatory reactions, and ameliorating fibrotic changes. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Yang, C.-H.; Tung, M.-C.; Lin, Y.-S.; Hsu, C.-Y.; Akhrymuk, I.; Tan, K.-T.; Ou, Y.-C.; Lin, C.-C. Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study. Life 2022, 12, 1693. | en |
| dc.identifier.doi | https://doi.org/10.3390/life12111693 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/112294 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | animal | en |
| dc.subject | anti-infective agents/therapeutic use | en |
| dc.subject | amnion/transplantation | en |
| dc.subject | cystitis | en |
| dc.subject | interstitial/therapy | en |
| dc.subject | chorion transplantation | en |
| dc.title | Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study | en |
| dc.title.serial | Life | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |