Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study

dc.contributor.authorYang, Che-Hsuehen
dc.contributor.authorTung, Min-Cheen
dc.contributor.authorLin, Yi-Shengen
dc.contributor.authorHsu, Chao-Yuen
dc.contributor.authorAkhrymuk, Ivanen
dc.contributor.authorTan, Kok-Tongen
dc.contributor.authorOu, Yen-Chuanen
dc.contributor.authorLin, Chi-Chienen
dc.date.accessioned2022-10-26T19:28:07Zen
dc.date.available2022-10-26T19:28:07Zen
dc.date.issued2022-10-25en
dc.date.updated2022-10-26T11:07:54Zen
dc.description.abstract<b> </b>The study aimed to investigate the therapeutic impact of intravesical instillation of dehydrated human amnion-chorion membrane (HACM) extracts based on the primary pathological feature of interstitial cystitis (IC). We divided 15 female Sprague-Dawley rats into three groups: sham control, IC, and treatment group. IC was induced by 400-&micro;L lipopolysaccharide (1 &micro;g/&micro;L), and it was replaced with normal saline in the sham control group. After IC induction, 300 &micro;L dehydrated HACM extracts (3 mg/kg) were instilled into rats&rsquo; urinary bladder weekly for 3 weeks. General histology, inflammatory cytokines, NF-&kappa;B, oxidative markers, and western blots results were examined. The urothelial denudation, mast-cell infiltration, and tissues fibrosis were all ameliorated. The elevated TNF-&alpha;, IL-1&beta;, IL-6, IL-8, and NF-&kappa;B were all down-regulated by dehydrated HACM extracts (<i>p</i> &lt; 0.05). For reactive oxygen species, increased malondialdehyde, decreased superoxide dismutase, and decreased glutathione peroxidase were all reversed (<i>p</i> &lt; 0.05). In apoptosis of IC, elevated Bax and suppressed Bcl-2 were improved (<i>p</i> &lt; 0.05) after instillation. In fibrosis, dysregulated TGF&beta;/R-Smads/Snail was corrected by the instillation of dehydrated HACM (<i>p</i> &lt; 0.05). In conclusion, dehydrated HACM extracts could be a powerful remedy in treating IC by reconstructing the damaged urothelium, reducing mast-cell infiltration and inflammatory reactions, and ameliorating fibrotic changes.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationYang, C.-H.; Tung, M.-C.; Lin, Y.-S.; Hsu, C.-Y.; Akhrymuk, I.; Tan, K.-T.; Ou, Y.-C.; Lin, C.-C. Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study. Life 2022, 12, 1693.en
dc.identifier.doihttps://doi.org/10.3390/life12111693en
dc.identifier.urihttp://hdl.handle.net/10919/112294en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleDehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Studyen
dc.title.serialLifeen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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