Mutagenesis of nifE and nifN from Azotobacter vinelandii

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dc.contributor.authorWilson, Mark Steven Michaelen
dc.contributor.committeechairDean, Dennis R.en
dc.contributor.committeememberSitz, Thomas O.en
dc.contributor.committeememberChen, Jiann-Shinen
dc.contributor.departmentAnaerobic Microbiologyen
dc.date.accessioned2014-03-14T21:37:43Zen
dc.date.adate2012-06-10en
dc.date.available2014-03-14T21:37:43Zen
dc.date.issued1988-12-15en
dc.date.rdate2012-06-10en
dc.date.sdate2012-06-10en
dc.description.abstractThe products of nifE and nifN from Azotobacter vinelandii, which are involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co) co) from nitrogenase, have been analyzed using a variety of mutagenic techniques. NifE was the object of several site-specific, amino acid substitutions that were designed to elicit information regarding metal cluster ligands, subunit-subunit interactions, and the proposed transfer of FeMo-co.from a nifEN-products complex to the apo-MoFe protein. A model of metal cluster binding; regions within the nifEN-products is discussed insofar as it relates to the rationale for the targeting of particular amino acids for-substitution. A translational fusion between nifN and lacZ was constructed and used to study the regulation of nifEN. This gene fusion was regulated in the same manner as wild type nifN and produced a fusion protein which was enzymatically active with respect to substrates of β-galactosidase. Results from mutant strains which carry lesions in nifH or nifA in addition to the nifNen
dc.description.degreeMaster of Scienceen
dc.format.extentvii, 64 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06102012-040400en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06102012-040400/en
dc.identifier.urihttp://hdl.handle.net/10919/43071en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.W552.pdfen
dc.relation.isformatofOCLC# 19570337en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.W552en
dc.subject.lcshNitrogen -- Fixationen
dc.subject.lcshNitrogen-fixing microorganismsen
dc.titleMutagenesis of nifE and nifN from Azotobacter vinelandiien
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineAnaerobic Microbiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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