Oxytetracycline and Monensin Uptake by Tifton 85 Bermudagrass from Dairy Manure-Applied Soil

TR Number
Date
2020-03-27
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract

To address concerns regarding the potential impact of antibiotic use in animal husbandry on antibiotic resistance in humans, we conducted a greenhouse-based study examining uptake of the veterinary antibiotics oxytetracycline (OTC) and monensin (MON) by Tifton 85 Bermudagrass (T85), the most commonly grown forage grass in the southeastern U.S.A. Since oxytetracycline is used in both veterinary and human medicine, its accumulation in animal products could impact human resistance to this antibiotic. Monensin is not used in human medicine but has a high potential for accumulating in the environment. Our research examined antibiotic uptake by forage grass T85, the effect of dairy manure application on its uptake, and antibiotic retention in soil. We compared unspiked, wet dairy manure to wet dairy manure spiked with MON or OTC that was soil surface applied to pots or incorporated into soil. After 6 wk, plant stem/leaf and root tissue, as well as soil samples, were assessed for antibiotic residues using enzyme-linked immunosorbent assay (ELISA). Results confirmed Tifton 85 MON and OTC uptake. Six weeks after adding the antibiotics, the greatest plant matter OTC and MON contents were 157.9 ± 70.6 and 234.4 ± 19.6 µg kg−1, respectively, and 17.6 and 369.5 µg kg−1, respectively, for soil. When spiked with OTC, manure incorporation led to decreased OTC uptake by T85 tissue. Bioaccumulation of these antimicrobials in livestock and in the environment is a potential concern for animal, environmental, and human health.

Description
Keywords
antibiotics, monensin, oxytetracycline, Tifton 85, dairy manure, ELISA, surface application, incorporation, forage
Citation
Hilaire, S.S.; Bellows, B.; Brady, J.A.; Muir, J.P. Oxytetracycline and Monensin Uptake by Tifton 85 Bermudagrass from Dairy Manure-Applied Soil. Agronomy 2020, 10, 468.