A model of the checkpoint response of the cell cycle of frog-egg extracts in the presence of unreplicated DNA

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dc.contributor.authorDravid, Amiten
dc.contributor.committeechairTyson, John J.en
dc.contributor.committeecochairHerdman, Terry L.en
dc.contributor.committeememberSible, Jill C.en
dc.contributor.committeememberBorggaard, Jeffrey T.en
dc.contributor.departmentMathematicsen
dc.date.accessioned2014-03-14T20:50:03Zen
dc.date.adate2004-12-22en
dc.date.available2014-03-14T20:50:03Zen
dc.date.issued2004-12-09en
dc.date.rdate2006-12-22en
dc.date.sdate2004-12-16en
dc.description.abstractThe cell cycle of eukaryotes consists of alternation between growth and DNA replication (interphase), and DNA distribution and cell-division (mitosis or M-phase). This process is regulated by a complex network of biochemical reactions. A core part of this network, called the "Cell Cycle engine" is evolutionarily conserved. The dimer of CDK1 (a protein kinase) and Cyclin proteins (the regulatory components), called M-phase Promoting Factor (MPF), and its key regulatory proteins Cdc25 and Wee1, are central parts of this cell cycle engine. Maintaining the fidelity of the DNA during the cell cycle is critical for successful propagation of the cell lineage. In the presence of unreplicated DNA, the cell cycle engine''s progress into mitosis is slowed down (or halted) by regulation of MPF activity through Cdc25 and Wee1. This regulatory event, called the unreplicated DNA checkpoint, was modeled in a rudimentary fashion in the Novak and Tyson (1993) model of frog eggs. Since then, many new experiments have uncovered relevant parts of this network. Here, we include these parts into a detailed model of the unreplicated DNA checkpoint in the cell cycle of frog-egg extracts. This work and future studies of the unreplicated DNA checkpoint will lead to its better understanding and hopefully to some strategies for tackling cancer.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-12162004-112420en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12162004-112420/en
dc.identifier.urihttp://hdl.handle.net/10919/36191en
dc.publisherVirginia Techen
dc.relation.haspartmsthesis_main.pdfen
dc.relation.haspartchecklist_for_fair_use_edt.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcell cycleen
dc.subjectcheckpointen
dc.subjectunreplicated DNAen
dc.subjectordinary differential equationsen
dc.subjectChk1en
dc.subjectwiring diagramen
dc.subjectcomputational modelingen
dc.subjectfrog egg extractsen
dc.titleA model of the checkpoint response of the cell cycle of frog-egg extracts in the presence of unreplicated DNAen
dc.typeThesisen
thesis.degree.disciplineMathematicsen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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