Electrochemical Urea Biosensor Based on Sol-gel Derived Nanostructured Cerium Oxide

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dc.contributor.authorAnsari, Anees A.en
dc.contributor.authorAli, Md. Azaharen
dc.contributor.authorMalhotra, B. D.en
dc.date.accessioned2023-01-20T15:46:29Zen
dc.date.available2023-01-20T15:46:29Zen
dc.date.issued2012-04-18en
dc.date.updated2023-01-19T22:29:59Zen
dc.description.abstractUrease (Urs) and glutamate dehydrogenase (GLDH) have been co-immobilized onto a nanostructured–cerium oxide (Nano-CeO2) film deposited onto a indium-tin-oxide (ITO) coated glass substrate by dip-coating via sol-gel process for urea detection. This nanostructured film has characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR), Scanning electron microscope (SEM) and electrochemical techniques, respectively. The particle size of the Nano-CeO2 film has been found to be 23 nm. Electrochemcial response (CV) studies show that Ur-GLDH/Nano-CeO2/ITO bioelectrode is found to be sensitive in the 10–80 mg/dL urea concentration range and can detect urea concentration upto 0.1 mg/dL level. The value of Michaelis–Menten constant (Km) estimated using Lineweaver–Burke plot found as 6.09 mg/dL indicates enhancement in the affinity and/or activity of enzyme attached to their nanobiocomposite. This bioelectrode retained 95% of enzyme activity after 6 months at 4°C.en
dc.description.notesYes, full paper (Peer reviewed?)en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1088/1742-6596/358/1/012006en
dc.identifier.eissn1742-6596en
dc.identifier.issn2010-1945en
dc.identifier.issue1en
dc.identifier.urihttp://hdl.handle.net/10919/113306en
dc.identifier.volume358en
dc.language.isoenen
dc.publisherWorld Scientificen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleElectrochemical Urea Biosensor Based on Sol-gel Derived Nanostructured Cerium Oxideen
dc.title.serialJournal of Physics: Conference Seriesen
dc.typeConference proceedingen
dc.type.dcmitypeTexten
pubs.finish-date2011-12-10en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Animal Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.start-date2011-12-07en

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