Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings

dc.contributor.authorCicek, Muzafferen
dc.contributor.committeechairWinkel, Brenda S. J.en
dc.contributor.committeememberRutherford, Charles L.en
dc.contributor.committeememberWalker, Roberten
dc.contributor.departmentBiologyen
dc.date.accessioned2014-03-14T21:50:11Zen
dc.date.adate2008-11-18en
dc.date.available2014-03-14T21:50:11Zen
dc.date.issued1996-02-04en
dc.date.rdate2008-11-18en
dc.date.sdate2008-11-18en
dc.description.abstractA full-length β-glucosidase encoding cDNA is isolated and sequenced from Sorghum bicolor (L) Moench. Using 5’- and 3’- end specific probes derived from the cDNA clone, the multiplicity of β-glucosidase genes and their expression in different tissues were studied. Southern blotting data showed that β-glucosidase is encoded by a small multigene family. Northern analysis data indicated that mRNA corresponding to the cloned gene is present at high levels in the node and mesocotyl 2 regions of the seedling and at low levels only in the zone of elongation region in roots. Other seedling parts such as mesocotyl 1, root sections adjacent to the seed and coleoptile sections do not have any detectable expression. The amino acid sequence data show 72% sequence identity between maize and sorghum β-glucosidase precursor proteins. In view of high sequence similarity between maize and sorghum β-glucosidase, immunoblotting analysis was performed with maize-anti-β-glucosidase serum. The immunoblotting results supported the results of Hosel et al., (1987) with respect to the occurrence of two distinct β-glucosidases being present in sorghum.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 68 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11182008-063432en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11182008-063432/en
dc.identifier.urihttp://hdl.handle.net/10919/45861en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1996.C534.pdfen
dc.relation.isformatofOCLC# 34929170en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectgene expressionen
dc.subjectdhurrinaseen
dc.subject.lccLD5655.V855 1996.C534en
dc.titleCloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlingsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen
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