Isolation of high-quality total RNA and RNA sequencing of single bovine oocytes

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dc.contributor.authorBiase, Fernando H.en
dc.date.accessioned2022-01-03T14:18:23Zen
dc.date.available2022-01-03T14:18:23Zen
dc.date.issued2021-12en
dc.date.updated2022-01-03T14:18:18Zen
dc.description.abstractStudying individual mammalian oocytes has been extremely valuable for the understanding of the molecular composition of oocytes including RNA storage. Here, a detailed protocol for isolation of oocytes, extraction of total RNA from single oocytes followed by full-length cDNA amplification, and library preparation is presented. The procedure permits the production of cost-effective and high-quality sequencing libraries. This protocol can be adapted for transcriptome analysis of oocytes from other species and be used to generate high-quality data from single embryos. For complete details on the use and execution of this protocol, please refer to Biase and Kimble (2018).en
dc.description.versionPublished versionen
dc.format.extentPages 100895en
dc.format.mimetypeapplication/pdfen
dc.identifier100895 (Article number)en
dc.identifier.doihttps://doi.org/10.1016/j.xpro.2021.100895en
dc.identifier.eissn2666-1667en
dc.identifier.issn2666-1667en
dc.identifier.issue4en
dc.identifier.orcidBiase, Fernando [0000-0001-7895-0223]en
dc.identifier.otherPMC8536786en
dc.identifier.otherS2666-1667(21)00601-8 (PII)en
dc.identifier.pmid34723212en
dc.identifier.urihttp://hdl.handle.net/10919/107310en
dc.identifier.volume2en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/34723212en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBioinformaticsen
dc.subjectCell isolationen
dc.subjectGene Expressionen
dc.subjectMolecular Biologyen
dc.subjectRNAseqen
dc.subjectSequencingen
dc.subjectSingle Cellen
dc.titleIsolation of high-quality total RNA and RNA sequencing of single bovine oocytesen
dc.title.serialSTAR protocolsen
dc.typeProtocolen
dc.type.dcmitypeTexten
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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