Quantifying rates of random mating in western corn rootworm emerging from Cry3Bb1-expressing and refuge maize in field cages

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dc.contributor.authorTaylor, Sally V.en
dc.contributor.authorSmith, Steven J.en
dc.contributor.authorKrupke, Christian H.en
dc.contributor.departmentEntomologyen
dc.date.accessioned2016-12-19T19:59:15Zen
dc.date.available2016-12-19T19:59:15Zen
dc.date.issued2016-12-08en
dc.description.abstractThe western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is the most significant pest of field maize, Zea mays L. (Poaceae), in the USA. Maize plants expressing Bt toxins targeting the corn rootwormcomplex have been widely adopted and are the primary insecticidal control measure for this pest in North America. Insect resistance management tactics using various refuge structures have been adopted to ensure Bt products will retain durability. An assumption of the refuge strategy is that males and females emerging from Bt and refuge plantings mate randomly; this has not been tested in the field. We conducted cage studies using field populations of WCR in Indiana, USA, to generate empirical field data on mating rates between beetles emerging from Cry3Bb1-expressing Bt and refuge maize plants. Two refuge configurations were tested; all refuge plants were labeled using the stable isotope 15N. This mark persists in adult beetles after eclosion, allowing for collection and analysis of isotopic ratios of all beetles. Additional data collected included adult emergence rates, timing and sex ratios for each of the treatments, and head capsule size and dry weights of beetles collected. Treatment had a significant effect on dry weight; mean dry weight decreased in Bt-only treatments. Fisher’s exact test of proportions of mating pairs of refuge and Bt insects indicated that mating was not random in 20% strip refuges and 5% seed blend treatments.We found high percentages of beetles that fed on Bt-expressing plants as larvae, suggesting thatmating between resistant beetles may not be rare even if randommating did occur.en
dc.description.versionPublished versionen
dc.format.extent203 - 212 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.issue3en
dc.identifier.urihttp://hdl.handle.net/10919/73728en
dc.identifier.volume161en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleQuantifying rates of random mating in western corn rootworm emerging from Cry3Bb1-expressing and refuge maize in field cagesen
dc.title.serialEntomologia Experimentalis et Applicataen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Tidewater ARECen
pubs.organisational-group/Virginia Tech/All T&R Facultyen

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