An artificial larval medium for colonized Culicoides guttipennis (coguillett) (Diptera: Ceratopogonidae)

dc.contributor.authorWilliams, Ralph E.en
dc.contributor.departmentEntomologyen
dc.date.accessioned2014-03-14T21:36:27Zen
dc.date.adate2010-05-19en
dc.date.available2014-03-14T21:36:27Zen
dc.date.issued1974en
dc.date.rdate2010-05-19en
dc.date.sdate2010-05-19en
dc.description.abstractAn artificial larval medium for colonized Culicoides guttipennis was developed. Microorganisms in decaying leaf matter were present in the original laboratory diet. These were believed to be the main food of C. guttipennis larvae. This was verified by sterilizing the leaf matter to kill the organisms present. Larvae did not develop beyond the first instar in this medium, but adults were produced from non-sterilized leaf matter. The objective was to develop cultures of various microorganisms and find one that would be best for rearing C. guttipennis larvae in producing more adults. A simple hay-infusion medium inoculated with stump-hole microorganisms was found more effective than all other cultures screened in rearing the larvae. This culture consisted of stump-hole microorganisms inoculated fresh each week from stored stumphole contents. It was compared with the same culture containing stumphole microorganisms completely adapted to a laboratory environment. The former was better in rearing C. guttipennis larvae. A final experiment evaluated the incorporation of vermiculite as an artificial substrate. This proved successful in rearing larvae and producing large numbers of adults. This culture was superior over the larval rearing medium of decaying leaf matter. Development of a successful artificial larval medium for colonized C. guttipennis will aid in establishing other laboratory colonies of related Culicoides species.en
dc.description.degreeMaster of Scienceen
dc.format.extent107 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-05192010-020059en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05192010-020059/en
dc.identifier.urihttp://hdl.handle.net/10919/42743en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1974.W55.pdfen
dc.relation.isformatofOCLC# 21813939en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1974.W55en
dc.subject.lcshCeratopogonidae -- Larvaeen
dc.subject.lcshCulicoides -- Larvaeen
dc.titleAn artificial larval medium for colonized Culicoides guttipennis (coguillett) (Diptera: Ceratopogonidae)en
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineEntomologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
LD5655.V855_1974.W55.pdf
Size:
5.87 MB
Format:
Adobe Portable Document Format
Collections