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A conserved viral amphipathic helix governs the replication site-specific membrane association

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dc.contributor.authorSathanantham, Preethien
dc.contributor.authorZhao, Wenhaoen
dc.contributor.authorHe, Guijuanen
dc.contributor.authorMurray, Austinen
dc.contributor.authorFenech, Emmaen
dc.contributor.authorDiaz, Arturoen
dc.contributor.authorSchuldiner, Mayaen
dc.contributor.authorWang, Xiaofengen
dc.date.accessioned2023-01-19T18:31:01Zen
dc.date.available2023-01-19T18:31:01Zen
dc.date.issued2022-09-01en
dc.date.updated2023-01-19T05:42:36Zen
dc.description.abstractPositive-strand RNA viruses assemble their viral replication complexes (VRCs) on specific host organelle membranes, yet it is unclear how viral replication proteins recognize and what motifs or domains in viral replication proteins determine their destinations. We show here that an amphipathic helix, helix B in replication protein 1a of brome mosaic virus (BMV), is necessary for 1a s localization to the nuclear endoplasmic reticulum (ER) membrane where BMV assembles its VRCs. Helix B is also sufficient to target soluble proteins to the nuclear ER membrane in yeast and plant cells. We further show that an equivalent helix in several plant-and human-infecting viruses of the Alsuviricetes class targets fluorescent proteins to the organelle membranes where they form their VRCs, including ER, vacuole, and Golgi membranes. Our work reveals a conserved helix that governs the localization of VRCs among a group of viruses and points to a possible target for developing broad-spectrum antiviral strategies.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1010752en
dc.identifier.eissn1553-7374en
dc.identifier.issn1553-7366en
dc.identifier.issue9en
dc.identifier.orcidWang, Xiaofeng [0000-0002-3850-3274]en
dc.identifier.otherPPATHOGENS-D-21-02576 (PII)en
dc.identifier.pmid36048900en
dc.identifier.urihttp://hdl.handle.net/10919/113267en
dc.identifier.volume18en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/36048900en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectInfectious Diseasesen
dc.subjectInfectionen
dc.subject.meshEndoplasmic Reticulumen
dc.subject.meshHumansen
dc.subject.meshBromovirusen
dc.subject.meshSaccharomyces cerevisiaeen
dc.subject.meshViral Proteinsen
dc.subject.meshRNA, Viralen
dc.subject.meshVirus Replicationen
dc.titleA conserved viral amphipathic helix governs the replication site-specific membrane associationen
dc.title.serialPLoS Pathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2022-07-19en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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