Cryopreservation of eukaryote algae

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dc.contributor.authorBeaty, Myron H.en
dc.contributor.committeechairParker, Bruce C.en
dc.contributor.committeememberMiller, Orson K. Jr.en
dc.contributor.committeememberOrcutt, David M.en
dc.contributor.departmentBiologyen
dc.date.accessioned2014-03-14T20:42:08Zen
dc.date.adate2008-07-28en
dc.date.available2014-03-14T20:42:08Zen
dc.date.issued1991-12-01en
dc.date.rdate2008-07-28en
dc.date.sdate2008-07-28en
dc.description.abstractIn an attempt to expand the knowledge of the diversity of algal taxa that are capable of successful recovery after freezing to and thawing from -196°C, 365 algal strains representing 13 algal classes were studied using various cryoprotectants at several concentrations. A cryopreservation protocol involving culturing the algae under low light (17-22 μE m⁻² s⁻¹) on semisolid (I % agar) media, controlled cooling at 1°C min⁻¹, rapid thawing, and the return of cultures to agar media was developed. This protocol produced successful recovery of >77% (283) of the algal strains tested. Of the 283 successfully cryopreserved strains comprising 240 species (68 genera), >220 species (34 genera) were cryopreserved for the first time. In addition, preliminary data suggest that some relationships exist among proposed systematic evolutionary lines, environmental preferences, cryoprotectants, and the organism's cryopreservability.en
dc.description.degreeMaster of Scienceen
dc.format.extent117 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07282008-135156en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07282008-135156/en
dc.identifier.urihttp://hdl.handle.net/10919/34224en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1991.B427.pdfen
dc.relation.isformatofOCLC# 25472497en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1991.B427en
dc.subject.lcshAlgaeen
dc.subject.lcshCryopreservation of organs, tissues, etcen
dc.titleCryopreservation of eukaryote algaeen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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