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An investigation of temperature effects on denitrifying bacterial populations in a biological nutrient removal (BNR) system

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dc.contributor.authorBrooks, Patrick C.en
dc.contributor.committeecochairLove, Nancy G.en
dc.contributor.committeecochairRandall, Clifford W.en
dc.contributor.committeememberBoardman, Gregory D.en
dc.contributor.departmentEnvironmental Engineeringen
dc.date.accessioned2014-03-14T21:31:00Zen
dc.date.adate2009-03-04en
dc.date.available2014-03-14T21:31:00Zen
dc.date.issued1996-04-02en
dc.date.rdate2009-03-04en
dc.date.sdate2009-03-04en
dc.description.abstractThe goal of this research was to characterize the effects of temperature changes on the denitrification process in a biological nutrient removal (BNR) system. Specifically, there were three objectives. First, the effects of temperature changes on denitrification rates by a bacterial population from a BNR system were investigated. Next, the role which PHAs (poly-beta-hydroxyalkanoates) played in the denitrification process were examined. Finally, the effect of temperature changes on the production and consumption rates of PHAs was determined. Sacrificial batch experiments were performed to assess the kinetic and chemical trends present in the denitrification process. Mixed liquor from the last anaerobic zone of a pilot scale BNR system was injected into vials. These vials were pre-purged with nitrogen gas in order to prevent dimolecular oxygen (02) from being entrained in the mixed liquor. Next, the vials were placed on a shaker table for 30 minutes in order to allow all external COD to be consumed. Following this, each vial was injected with nitrates and various macronutrients. This process was repeated for three different sets of batch tests; each set was identical except for the added substrate. One set received no added substrate while the other two received either acetate or glucose. Vials were sacrificed over a period of three hours and analyzed for nitrate, phosphate, PHB (polybeta-hydroxybutyrate), PHV (poly-beta-hydroxyvalerate), glucose and acetate.en
dc.description.degreeMaster of Scienceen
dc.format.extentx, 116 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-03042009-041300en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-03042009-041300/en
dc.identifier.urihttp://hdl.handle.net/10919/41429en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1996.B766.pdfen
dc.relation.isformatofOCLC# 34923201en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectbiological phosphorus removalen
dc.subjectpoly-betahydroxybutyrateen
dc.subjectdenitrificationen
dc.subject.lccLD5655.V855 1996.B766en
dc.titleAn investigation of temperature effects on denitrifying bacterial populations in a biological nutrient removal (BNR) systemen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineEnvironmental Planningen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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