Herbicide injury induces DNA methylome alterations in Arabidopsis

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dc.contributor.authorKim, Gunjuneen
dc.contributor.authorClarke, Christopher R.en
dc.contributor.authorLarose, Haileyen
dc.contributor.authorTran, Hong T.en
dc.contributor.authorHaak, David C.en
dc.contributor.authorZhang, Liqingen
dc.contributor.authorAskew, Shawn D.en
dc.contributor.authorBarney, Jacoben
dc.contributor.authorWestwood, James H.en
dc.contributor.departmentComputer Scienceen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2018-01-05T12:26:11Zen
dc.date.available2018-01-05T12:26:11Zen
dc.date.issued2017-07-20en
dc.description.abstractThe emergence of herbicide-resistant weeds is a major threat facing modern agriculture. Over 470 weedy-plant populations have developed resistance to herbicides. Traditional evolutionary mechanisms are not always sufficient to explain the rapidity with which certain weed populations adapt in response to herbicide exposure. Stress-induced epigenetic changes, such as alterations in DNA methylation, are potential additional adaptive mechanisms for herbicide resistance. We performed methylC sequencing of Arabidopsis thaliana leaves that developed after either mock treatment or two different sub-lethal doses of the herbicide glyphosate, the most-used herbicide in the history of agriculture. The herbicide injury resulted in 9,205 differentially methylated regions (DMRs) across the genome. In total, 5,914 of these DMRs were induced in a dose-dependent manner, wherein the methylation levels were positively correlated to the severity of the herbicide injury, suggesting that plants can modulate the magnitude of methylation changes based on the severity of the stress. Of the 3,680 genes associated with glyphosate-induced DMRs, only 7% were also implicated in methylation changes following biotic or salinity stress. These results demonstrate that plants respond to herbicide stress through changes in methylation patterns that are, in general, dose-sensitive and, at least partially, stress-specific.en
dc.description.versionPublished versionen
dc.format.extent? - ? (17) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.7717/peerj.3560en
dc.identifier.issn2167-8359en
dc.identifier.orcidHaak, DC [0000-0002-3692-3152]en
dc.identifier.orcidAskew, S [0000-0002-9515-9502]en
dc.identifier.orcidBarney, J [0000-0003-2949-5003]en
dc.identifier.urihttp://hdl.handle.net/10919/81544en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherPeerJen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406374200006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectmethylkiten
dc.subjectarabidopsisen
dc.subjectglyphosateen
dc.subjectherbicide resistanceen
dc.subjectcytosine dna methylationen
dc.subjectmethylc-seqen
dc.subjectglyphosate resistanceen
dc.subjectepigenetic variationen
dc.subjectconyza-canadensisen
dc.subjectmethylationen
dc.subjectthalianaen
dc.subjectgenomeen
dc.subjectmethyltransferasesen
dc.subjectpopulationen
dc.subjectevolutionen
dc.subjectresponsesen
dc.titleHerbicide injury induces DNA methylome alterations in Arabidopsisen
dc.title.serialPeerJen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Plant Pathology, Physiology, & Weed Scienceen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen

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