Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs

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dc.contributor.authorZhang, Husenen
dc.contributor.authorWang, Haifengen
dc.contributor.authorShepherd, Megan L.en
dc.contributor.authorWen, Keen
dc.contributor.authorLi, Guohuaen
dc.contributor.authorYang, Xingdongen
dc.contributor.authorKocher, Jacoben
dc.contributor.authorGiri-Rachman, Ernawatien
dc.contributor.authorDickerman, Allan W.en
dc.contributor.authorSettlage, Robert E.en
dc.contributor.authorYuan, Lijuanen
dc.contributor.departmentCivil and Environmental Engineeringen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2014-09-24T11:13:18Zen
dc.date.available2014-09-24T11:13:18Zen
dc.date.issued2014-09-09en
dc.date.updated2014-09-24T11:13:19Zen
dc.description.abstractWe generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of Lactobacillus rhamnosus GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of Enterococcus in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota’s response to probiotic interventions for treating infantile HRV infection.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGut Pathogens. 2014 Sep 09;6(1):39en
dc.identifier.doihttps://doi.org/10.1186/s13099-014-0039-8en
dc.identifier.urihttp://hdl.handle.net/10919/50540en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderHusen Zhang et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleProbiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigsen
dc.title.serialGut Pathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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