Plasmid-influenced changes in Mycobacterium avium catalase activity

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dc.contributor.authorPethel, Michele Leeen
dc.contributor.departmentMicrobiologyen
dc.date.accessioned2014-03-14T21:38:23Zen
dc.date.adate2010-06-12en
dc.date.available2014-03-14T21:38:23Zen
dc.date.issued1988en
dc.date.rdate2010-06-12en
dc.date.sdate2010-06-12en
dc.description.abstractA virulent Mycobacterium avium strain, LR25, which carries 3 plasmids (18, 28, and 165 kb) and grows at 43°C was compared to its plasmid-free, avirulent segregant, strain LR163, to investigate the basis for the latter's inability to grow at 43°C. The failure of mid-log phase cultures of strain LR163 to grow at 43°C was dependent upon the presence of high levels of culture aeration. In addition, highly aerated mid-log phase cultures of strain LR163 failed to grow at 379C, By contrast, late-log phase cultures of strain LR163 were capable of growth when shifted to 43°9C under highly aerobie conditions. Mid-log phase cells of strain LR163 had 30% of the catalase activity of mid-log phase cells of strain LR25 and were more susceptible to hydrogen peroxide (0.08% w/v). Catalase activities of late-log, early-stationary, and stationary phase cells of strain LR163 were Significantly higher than mid-log phase cells. Catalase activity of strain LR25 was highest in cells of mid-log phase cultures, whereas the catalase activity of strain LR163 was highest in cells of stationary phase cultures. These data support the idea that plasmid-encoded genes influence M. avium catalase activity.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 68 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06122010-020434en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06122010-020434/en
dc.identifier.urihttp://hdl.handle.net/10919/43266en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.P384.pdfen
dc.relation.isformatofOCLC# 19570273en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.P384en
dc.subject.lcshMycobacteriumen
dc.subject.lcshPlasmidsen
dc.titlePlasmid-influenced changes in Mycobacterium avium catalase activityen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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