Gene Expression of the Intestinal Peptide Transporter 1 and Peroxisome Proliferator-Activated Receptor alpha Following Fasting/Refeeding and Ligand Administration in Chickens

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dc.contributor.authorMadsen, Sara Lindslyen
dc.contributor.committeechairWong, Eric A.en
dc.contributor.committeememberDenbow, D. Michaelen
dc.contributor.committeememberMcElroy, Audrey P.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2014-03-14T20:50:43Zen
dc.date.adate2010-01-12en
dc.date.available2014-03-14T20:50:43Zen
dc.date.issued2009-12-11en
dc.date.rdate2010-01-12en
dc.date.sdate2009-12-23en
dc.description.abstractThe uptake of amino acids is mediated by amino acid transporters and the peptide transporter, PepT1. The nuclear receptor PPARα may play a role in the activation of PepT1. The objectives of this study were to evaluate the effect of a fasting/refeeding regimen and gavaging a PPARα ligand on expression of chicken PepT1 and PPARα. During the fasting/refeeding trial, chicks were placed on a 24h fast and then divided into 3 groups (Continuously Fasted (CF), Refed/Food Withdrawn, and Refed Adlib) and sampled 1, 2, 3, 5 and 7h post-fast. Expression of PepT1 and PPARα increased almost 2-fold post-24h fast (P < 0.002). The CF group had highest expression for both genes (P < 0.0001) suggesting a coordinate change in PepT1 and PPARα expression during fasting, indicating a possible regulatory role for PPARα of PepT1. Oral gavage trials utilizing PPARα ligand WY- 14643 were conducted with layers and broilers. For the layer trial birds were gavaged daily for 3 d with 50 mg WY-14643/kg in carrier (Ligand) or with just carrier (No Ligand) and sampled on d4. For the broiler trial, birds were gavaged daily with 25 or 50 mg WY-14643/kg or just carrier and sampled on d2 or d3 post gavage. No difference in PepT1 was observed between the Ligand and No Ligand groups. In the layer trial, PPARα decreased 6-fold (P = 0.005) in the Ligand group. Broilers treated with WY-14643 showed no effect of ligand. These results indicate a difference between layers and broilers in response to WY-14643.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-12232009-114026en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12232009-114026/en
dc.identifier.urihttp://hdl.handle.net/10919/36425en
dc.publisherVirginia Techen
dc.relation.haspartMadsen_SL_T_2009.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPepT1en
dc.subjectFastingen
dc.subjectChickenen
dc.subjectPPARαen
dc.titleGene Expression of the Intestinal Peptide Transporter 1 and Peroxisome Proliferator-Activated Receptor alpha Following Fasting/Refeeding and Ligand Administration in Chickensen
dc.typeThesisen
thesis.degree.disciplineAnimal and Poultry Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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