Chromosome-level genome assemblies of the malaria vectors Anopheles coluzzii and Anopheles arabiensis

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dc.contributor.authorZamyatin, Antonen
dc.contributor.authorAvdeyev, Pavelen
dc.contributor.authorLiang, Jiangtaoen
dc.contributor.authorSharma, Atashien
dc.contributor.authorChen, Chujiaen
dc.contributor.authorLukyanchikova, Varvaraen
dc.contributor.authorAlexeev, Nikitaen
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorAlekseyev, Max A.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2021-08-24T13:28:22Zen
dc.date.available2021-08-24T13:28:22Zen
dc.date.issued2021-03-01en
dc.date.updated2021-08-24T13:28:18Zen
dc.description.abstractBackground: Anopheles coluzzii and Anopheles arabiensis belong to the Anopheles gambiae complex and are among the major malaria vectors in sub-Saharan Africa. However, chromosome-level reference genome assemblies are still lacking for these medically important mosquito species. Findings: In this study, we produced de novo chromosome-level genome assemblies for A. coluzzii and A. arabiensis using the long-read Oxford Nanopore sequencing technology and the Hi-C scaffolding approach. We obtained 273.4 and 256.8 Mb of the total assemblies for A. coluzzii and A. arabiensis, respectively. Each assembly consists of 3 chromosome-scale scaffolds (X, 2, 3), complete mitochondrion, and unordered contigs identified as autosomal pericentromeric DNA, X pericentromeric DNA, and Y sequences. Comparison of these assemblies with the existing assemblies for these species demonstrated that we obtained improved reference-quality genomes. The new assemblies allowed us to identify genomic coordinates for the breakpoint regions of fixed and polymorphic chromosomal inversions in A. coluzzii and A. arabiensis. Conclusion: The new chromosome-level assemblies will facilitate functional and population genomic studies in A. coluzzii and A. arabiensis. The presented assembly pipeline will accelerate progress toward creating high-quality genome references for other disease vectors.en
dc.description.versionPublished versionen
dc.format.extent16 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN giab017 (Article number)en
dc.identifier.doihttps://doi.org/10.1093/gigascience/giab017en
dc.identifier.eissn2047-217Xen
dc.identifier.issn2047-217Xen
dc.identifier.issue3en
dc.identifier.orcidLiang, Jiangtao [0000-0002-8468-7457]en
dc.identifier.orcidTu, Zhijian [0000-0003-4227-3819]en
dc.identifier.orcidSharakhov, Igor [0000-0003-0752-3747]en
dc.identifier.other6170950 (PII)en
dc.identifier.pmid33718948 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/104695en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000637267600008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectBiologyen
dc.subjectAnopheles arabiensisen
dc.subjectAnopheles coluzziien
dc.subjectmalaria mosquitoen
dc.subjectgenome assemblyen
dc.subjectOxford nanopore sequencingen
dc.subjectHi-C chromosome conformation captureen
dc.titleChromosome-level genome assemblies of the malaria vectors Anopheles coluzzii and Anopheles arabiensisen
dc.title.serialGigascienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherData Paperen
dc.type.otherJournalen
dcterms.dateAccepted2021-01-23en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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