Rewiring of gene expression in circulating white blood cells is associated with pregnancy outcome in heifers (Bos taurus)

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dc.contributor.authorMoorey, Sarah E.en
dc.contributor.authorWalker, Bailey N.en
dc.contributor.authorElmore, Michelle F.en
dc.contributor.authorElmore, Joshua B.en
dc.contributor.authorRodning, Soren P.en
dc.contributor.authorBiase, Fernando H.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2021-01-29T15:24:05Zen
dc.date.available2021-01-29T15:24:05Zen
dc.date.issued2020-10-08en
dc.description.abstractInfertility is a challenging phenomenon in cattle that reduces the sustainability of beef production worldwide. Here, we tested the hypothesis that gene expression profiles of protein-coding genes expressed in peripheral white blood cells (PWBCs), and circulating micro RNAs in plasma, are associated with female fertility, measured by pregnancy outcome. We drew blood samples from 17 heifers on the day of artificial insemination and analyzed transcript abundance for 10,496 genes in PWBCs and 290 circulating micro RNAs. The females were later classified as pregnant to artificial insemination, pregnant to natural breeding or not pregnant. We identified 1860 genes producing significant differential coexpression (eFDR<0.002) based on pregnancy outcome. Additionally, 237 micro RNAs and 2274 genes in PWBCs presented differential coexpression based on pregnancy outcome. Furthermore, using a machine learning prediction algorithm we detected a subset of genes whose abundance could be used for blind categorization of pregnancy outcome. Our results provide strong evidence that transcript abundance in circulating white blood cells is associated with fertility in heifers.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-020-73694-wen
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.other16786en
dc.identifier.pmid33033295en
dc.identifier.urihttp://hdl.handle.net/10919/102131en
dc.identifier.volume10en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleRewiring of gene expression in circulating white blood cells is associated with pregnancy outcome in heifers (Bos taurus)en
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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