Epigenetic modifiers identified as regulators of food intake in a unique hypophagic chicken model

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dc.contributor.authorCao, Changen
dc.contributor.authorSiegel, Paul B.en
dc.contributor.authorGilbert, Elizabeth R.en
dc.contributor.authorCline, Mark A.en
dc.date.accessioned2022-12-14T15:31:08Zen
dc.date.available2022-12-14T15:31:08Zen
dc.date.issued2022-06en
dc.description.abstractDNA methylation is an epigenetic modification that influences gene transcription; however, the effects of methylation-influencing chemicals on appetite are unknown. We evaluated the effects of single administration of a methyl donor, S-Adenosylmethionine (SAM), or methylation inhibitor, 5-Azacytidine (AZA), on immediate and later-age food intake in an anorexic chick model. The doses of intracerebroventricularlyinjected SAM were 0 (vehicle), 0.1, 1, and 10 mu g, and of AZA were 0 (vehicle), 1, 5, and 25 mu g. When injected on day 5 posthatch, there was no effect of SAM on food intake in either fed or fasted chicks, whereas AZA increased food consumption in the fasted state but decreased it in fed chicks. We then performed a single injection (same doses) at hatch and measured food intake on day 5 in response to neuropeptide Y (NPY; 0.2 mu g) injection. Irrespective of NPY, chicks injected with 1 mu g of SAM ate more than others on day 5. In contrast, chicks injected with AZA (5 and 25 mu g doses) consumed less on day 5. In conclusion, we identified DNA methylation-regulating chemicals as regulators of food intake. AZA but not SAM affected food intake in the short-term, feeding state dependently. Later, both chemicals injected on the day of hatch were associated with food intake changes at a later age, suggesting that feeding pathways might be altered through changes in methylation. (c) 2022 The Author(s). Published by Elsevier B.V. on behalf of The Animal Consortium. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en
dc.description.notesThis project was supported by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture.en
dc.description.sponsorshipVirginia Agricultural Experiment Station; Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agricultureen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.animal.2022.100549en
dc.identifier.eissn1751-732Xen
dc.identifier.issn1751-7311en
dc.identifier.issue6en
dc.identifier.other100549en
dc.identifier.pmid35679817en
dc.identifier.urihttp://hdl.handle.net/10919/112883en
dc.identifier.volume16en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectS-Adenosylmethionineen
dc.subject5-Azacytidineen
dc.subjectAppetiteen
dc.subjectDNA methylationen
dc.subjectLow BW-selected chickenen
dc.titleEpigenetic modifiers identified as regulators of food intake in a unique hypophagic chicken modelen
dc.title.serialAnimalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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