Characterization of the Expression of Angiogenic Factors in Cutaneous Squamous Cell Carcinoma of Domestic Cats

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dc.contributor.authorGudenschwager-Basso, Erwin Kristobalen
dc.contributor.authorStevenson, Valentinaen
dc.contributor.authorSponenberg, D. Phillipen
dc.contributor.authorCecere, Thomas E.en
dc.contributor.authorHuckle, William R.en
dc.date.accessioned2022-07-26T13:24:06Zen
dc.date.available2022-07-26T13:24:06Zen
dc.date.issued2022-07-21en
dc.date.updated2022-07-25T16:32:28Zen
dc.description.abstractCutaneous squamous cell carcinoma (CSCC) is a common malignant skin cancer with a significant impact on health, and it is important to determine the degree of reliance of CSCC on angiogenesis for growth and metastasis. Major regulators of angiogenesis are the vascular endothelial growth factor (VEGF) family and their associated receptors. Alternative pre-mRNA splicing produces multiple isoforms of VEGF-A and PLGF with distinct biological properties. Several studies highlight the function of VEGF-A in CSCC, but there are no studies of the different isoforms of VEGF-A and PLGF for this neoplasm. We characterized the expression of three isoforms of VEGF-A, two isoforms of PLGF, and their receptors in cat CSCC biopsies compared to normal haired skin (NHS). Although our results revealed no significant changes in transcript levels of <i>panVEGF-A</i> or their isoforms, the mRNA levels of PLGF I and the receptors Flt-1 and KDR were downregulated in CSCC compared to NHS. Differences were observed in ligand:receptor mRNA expression ratio, with the expression of <i>VEGF-A</i> relative to its receptor <i>KDR</i> higher in CSCC, which is consistent with our hypothesis and prior human SCC studies. Immunolocalization in tissue showed increased expression of all measured factors and receptors in tumor cells compared to NHS and surrounding vasculature. We conclude that the factors measured may play a pivotal role in CSCC growth, although further studies are needed to clarify the role of angiogenic factors in feline CSCC.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGudenschwager-Basso, E.K.; Stevenson, V.; Sponenberg, D.P.; Cecere, T.E.; Huckle, W.R. Characterization of the Expression of Angiogenic Factors in Cutaneous Squamous Cell Carcinoma of Domestic Cats. Vet. Sci. 2022, 9, 375.en
dc.identifier.doihttps://doi.org/10.3390/vetsci9070375en
dc.identifier.urihttp://hdl.handle.net/10919/111368en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectVEGF-Aen
dc.subjectPLGFen
dc.subjectVEGFR1en
dc.subjectVEGFR2en
dc.subjectKDRen
dc.subjectFlt-1en
dc.subjectangiogenesisen
dc.subjectcutaneous squamous cell carcinomasen
dc.subjectcaten
dc.subjectfelineen
dc.titleCharacterization of the Expression of Angiogenic Factors in Cutaneous Squamous Cell Carcinoma of Domestic Catsen
dc.title.serialVeterinary Sciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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