Optical method of recording electrical activity in isolated rabbit hearts

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dc.contributor.authorAmanna, Ashwin E.en
dc.contributor.committeecochairKrauthamer, Victoren
dc.contributor.committeecochairReed, Jeffrey H.en
dc.contributor.committeememberWang, Anboen
dc.contributor.departmentElectrical Engineeringen
dc.date.accessioned2014-03-14T21:48:28Zen
dc.date.adate2009-10-31en
dc.date.available2014-03-14T21:48:28Zen
dc.date.issued1993-12-01en
dc.date.rdate2009-10-31en
dc.date.sdate2009-10-31en
dc.description.abstractA recently developed optical method utilizes a single, implantable, optical fiber to record electrical activity from isolated hearts stained with voltage-sensitive dyes. This optical technique generates recordings of transmembrane potential from excitable myocardial tissue, and remain free from stimulus artifacts that accompany electro stimulation and hinder all standard electrode recording methods during the application of high-voltage electrical shocks. The fiber optic system uses a l00J,lm diameter core fiber which can record from epicardial surface, internal mid-myocardium, or endocardial surfaces. The stained tissue is excited through the fiber and the resulting fluorescence is transmitted through the same fiber to a photomultiplier tube. Changes in fluorescence accompanying normal cardiac action potentials usually range from 1-5%. Substantive motion related signals accompany normal beating hearts, drowning out the actual signal that corresponds to change in membrane potential. When added to the nutrient solution of the heart, an excitation / contraction decoupler restricts motion and reduces the motion related signal making it easier to isolate the true membrane potential signal.en
dc.description.degreeMaster of Scienceen
dc.format.extentvii, 73 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-10312009-020219en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10312009-020219/en
dc.identifier.urihttp://hdl.handle.net/10919/45356en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1993.A445.pdfen
dc.relation.isformatofOCLC# 30505891en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1993.A445en
dc.subject.lcshEndoscopesen
dc.titleOptical method of recording electrical activity in isolated rabbit heartsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineElectrical Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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