Interbacterial Antagonism Mediated by a Released Polysaccharide

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dc.contributor.authorLiu, Yiweien
dc.contributor.authorGloag, Erin S.en
dc.contributor.authorHill, Preston J.en
dc.contributor.authorParsek, Matthew R.en
dc.contributor.authorWozniak, Daniel J.en
dc.contributor.editorEl-Naggar, Mohamed Y.en
dc.date.accessioned2022-12-14T20:53:38Zen
dc.date.available2022-12-14T20:53:38Zen
dc.date.issued2022-05en
dc.date.updated2022-12-14T19:08:05Zen
dc.description.abstractPseudomonas aeruginosa and Staphylococcus aureus are two common pathogens causing chronic infections in the lungs of people with cystic fibrosis (CF) and in wounds, suggesting that these two organisms coexist <i>in vivo</i>. However, P. aeruginosa utilizes various mechanisms to antagonize S. aureus when these organisms are grown together <i>in vitro</i>. Here, we suggest a novel role for Psl in antagonizing S. aureus growth. Psl is an exopolysaccharide that exists in both cell-associated and cell-free forms and is important for biofilm formation in P. aeruginosa. When grown in planktonic coculture with a P. aeruginosa <i>psl</i> mutant, S. aureus had increased survival compared to when it was grown with wild-type P. aeruginosa. We found that cell-free Psl was critical for the killing, as purified cell-free Psl was sufficient to kill S. aureus. Transmission electron microscopy of S. aureus treated with Psl revealed disrupted cell envelopes, suggesting that Psl causes S. aureus cell lysis. This was independent of known mechanisms used by P. aeruginosa to antagonize S. aureus. Cell-free Psl could also promote S. aureus killing during growth in <i>in vivo</i>-like conditions. We also found that Psl production in P. aeruginosa CF clinical isolates positively correlated with the ability to kill S. aureus. This could be a result of P. aeruginosa coevolution with S. aureus in CF lungs. In conclusion, this study defines a novel role for P. aeruginosa Psl in killing S. aureus, potentially impacting the coexistence of these two opportunistic pathogens <i>in vivo</i>. IMPORTANCE Pseudomonas aeruginosa and Staphylococcus aureus are two important opportunistic human pathogens commonly coisolated from clinical samples. However, P. aeruginosa can utilize various mechanisms to antagonize S. aureus <i>in vitro</i>. Here, we investigated the interactions between these two organisms and report a novel role for P. aeruginosa exopolysaccharide Psl in killing S. aureus. We found that cell-free Psl could kill S. aureus <i>in vitro</i>, possibly by inducing cell lysis. This was also observed in conditions reflective of <i>in vivo</i> scenarios. In accord with this, Psl production in P. aeruginosa clinical isolates positively correlated with their ability to kill S. aureus. Together, our data suggest a role for Psl in affecting the coexistence of P. aeruginosa and S. aureus <i>in vivo</i>.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1128/jb.00076-22en
dc.identifier.eissn1098-5530en
dc.identifier.issn0021-9193en
dc.identifier.issue5en
dc.identifier.orcidGloag, Erin [0000-0001-8895-3444]en
dc.identifier.otherPMC9112932en
dc.identifier.pmid35446119en
dc.identifier.urihttp://hdl.handle.net/10919/112893en
dc.identifier.volume204en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/35446119en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPseudomonas aeruginosaen
dc.subjectPslen
dc.subjectStaphylococcus aureusen
dc.subjectcell lysisen
dc.subjectcystic fibrosisen
dc.subjectexopolysaccharideen
dc.subjectpolymicrobialen
dc.subjectwounden
dc.subjectEmerging Infectious Diseasesen
dc.subjectRare Diseasesen
dc.subjectClinical Researchen
dc.subjectLungen
dc.subjectInfectious Diseasesen
dc.subject2.2 Factors relating to the physical environmenten
dc.subject2 Aetiologyen
dc.subjectInfectionen
dc.subjectCongenitalen
dc.subject.meshHumansen
dc.subject.meshBiofilmsen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshStaphylococcus aureusen
dc.subject.meshPseudomonas Infectionsen
dc.subject.meshStaphylococcal Infectionsen
dc.subject.meshCystic Fibrosisen
dc.subject.meshPolysaccharidesen
dc.subject.meshMicrobial Interactionsen
dc.titleInterbacterial Antagonism Mediated by a Released Polysaccharideen
dc.title.serialJournal of Bacteriologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherResearch Support, Non-U.S. Gov'ten
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
dc.type.otherResearch Support, N.I.H., Extramuralen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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