Dietary Supplementation of Algae-Derived Omega-3 Fatty Acids Influences Endometrial and Conceptus Transcript Profiles in Mares

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dc.contributor.authorKnight, James W.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2019-01-04T15:45:22Zen
dc.date.available2019-01-04T15:45:22Zen
dc.date.issued2018en
dc.date.updated2019-01-04T15:45:21Zen
dc.description.abstractMaternal diet has been shown to impact fetal development across a variety of species. Given the reported effects of omega-3 long-chain polyunsaturated fatty acid (n-3 LCPUFA) supplementation on health across species, it was hypothesized that supplementation of algal-based n-3 LCPUFA to mares around conception would alter the maternal uterine environment and modify gene expression in early conceptuses. Nonlactating, light horse mares were supplemented with concentrate (n = 6) or an isocaloric diet containing 0.06 g/kg body weight n-3 LCPUFA (primarily docosahexaenoic acid [DHA], n = 7) at least 60 days before first sample collection. Four consecutive ovulatory cycles were monitored. Uterine endometrial samples were obtained 12.5 days postovulation from cycles 1 (uninseminated), 3, and 4 (bred to a common stallion); embryos were flushed from cycles 3 and 4. Biopsies from supplemented mares were greater in DHA and total omega-3 fatty acid concentrations (P < .05), indicating successful tissue incorporation. Uninseminated n-3 LCPUFA supplemented mares displayed increased PTGFS and reduced IL6 gene expression (P < .01) than controls. Supplemented pregnant mares had increased mRNA abundance of PTGES (P = .05), PTGFS (P = .03), and SLCO2A1 (P = .01), while supplemented barren mares exhibited lower PTGFS (P = .05) and IL6 (P < .01) expression than control mares. In the conceptus, relative transcript abundance of trophoblast and endoderm markers (GATA3, GATA4, GATA6, TFAP2A, ELF3) was greater (P < .05) in embryos from supplemented mares. These results indicate algae-derived n-3 LCPUFA supplementation prior to breeding alters the maternal uterine environment and modifies expression of genes in preimplantation equine conceptusesen
dc.description.versionPublished versionen
dc.format.extentPages 66-75en
dc.format.extent10 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.orcidKnight, James [0000-0002-1707-0203]en
dc.identifier.urihttp://hdl.handle.net/10919/86602en
dc.identifier.volume62en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject0707 Veterinary Sciencesen
dc.subjectVeterinary Sciencesen
dc.titleDietary Supplementation of Algae-Derived Omega-3 Fatty Acids Influences Endometrial and Conceptus Transcript Profiles in Maresen
dc.title.serialJournal of Equine Veterinary Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dcterms.dateAccepted2017-08-01en
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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