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Expression of recombinant porcine preprorelaxin in Nicotiana tabacum

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dc.contributor.authorBuswell, Walter Scotten
dc.contributor.committeechairZhang, Chenming Mikeen
dc.contributor.committeememberJelesko, John G.en
dc.contributor.committeememberAgblevor, Foster Aryien
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2014-03-14T20:36:58Zen
dc.date.adate2006-06-14en
dc.date.available2014-03-14T20:36:58Zen
dc.date.issued2006-05-10en
dc.date.rdate2009-06-14en
dc.date.sdate2006-05-15en
dc.description.abstractRelaxin is a small peptide hormone that has demonstrated potential therapeutic actions for cardiovascular disease and fibrosis. Additionally, relaxin has demonstrated the ability to protect the heart from injuries caused by ischemia and reperfusion, promote the healing of ischemic ulcers, and counteract allergic responses. The objective of this research was to express fully processed porcine relaxin in transgenic tobacco plants, as an alternative to current methods of producing relaxin. Two recombinant relaxin genes were constructed that contained the patatin signal peptide cDNA fused in frame to prorelaxin cDNA, which was codon-optimized for expression in Nicotiana tabacum, under the control of either the "super" promoter or the dual enhanced cauliflower mosaic virus 35S promoter. Eighteen transgenic tobacco plants were generated that were transformed with the above recombinant genes. Preprorelaxin, mRNA was detected in 12 of the transgenic plants. Fully processed relaxin protein was not found in any tobacco plants that had demonstrated gene expression by northern blot analysis. Preprorelaxin was only identified in extracts from transgenic plants that contained the insoluble protein fraction, as determined by western blot analysis. Additionally, an increased yield of preprorelaxin was identified after incubation of tobacco leaves in an ubiquitin inhibitor.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05152006-130213en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05152006-130213/en
dc.identifier.urihttp://hdl.handle.net/10919/32803en
dc.publisherVirginia Techen
dc.relation.haspartThesis_Buswell.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectrecombinant proteinen
dc.subjecttransgenic tobaccoen
dc.subjectrelaxinen
dc.titleExpression of recombinant porcine preprorelaxin in Nicotiana tabacumen
dc.typeThesisen
thesis.degree.disciplineBiological Systems Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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