Discovery of Biofilm Inhibitors from the Microbiota of Marine Egg Masses

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dc.contributor.authorKyei, Loisen
dc.contributor.authorPiedl, Karlaen
dc.contributor.authorMiller, Eleanor M.en
dc.contributor.authorMevers, Emilyen
dc.date.accessioned2024-12-19T17:56:19Zen
dc.date.available2024-12-19T17:56:19Zen
dc.date.issued2024-05-30en
dc.description.abstractBiofilms commonly develop in immunocompromised patients, which leads to persistent infections that are difficult to treat. In the biofilm state, bacteria are protected against both antibiotics and the host’s immune system; currently, there are no therapeutics that target biofilms. In this study, we screened a chemical fraction library representing the natural product capacity of the microbiota of marine egg masses, namely, the moon snail egg collars. This led to the identification of active fractions targeting both Pseudomonas aeruginosa and Staphylococcus aureus biofilms. Subsequent analysis revealed that a subset of these fractions were capable of eradicating preformed biofilms, all against S. aureus. Bioassay-guided isolation led us to identify pseudochelin A, a known siderophore, as a S. aureus biofilm inhibitor with an IC50 of 88.5 μM. Mass spectrometry-based metabolomic analyses revealed widespread production of pseudochelin A among fractions possessing S. aureus antibiofilm properties. In addition, a key biosynthetic gene involved in producing pseudochelin A was detected on 30% of the moon snail egg collars and pseudochelin A is capable of inhibiting the formation of biofilms (IC50 50.6 μM) produced by ecologically relevant bacterial strains. We propose that pseudochelin A may have a role in shaping the microbiome or protecting the egg collars from microbiofouling.en
dc.description.versionPublished versionen
dc.format.extentPages 1635-1642en
dc.format.extent8 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1021/acs.jnatprod.4c00376en
dc.identifier.eissn1520-6025en
dc.identifier.issn0163-3864en
dc.identifier.issue6en
dc.identifier.orcidMevers, Emily [0000-0001-7986-5610]en
dc.identifier.pmid38814458en
dc.identifier.urihttps://hdl.handle.net/10919/123843en
dc.identifier.volume87en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/38814458en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject.meshAnimalsen
dc.subject.meshSnailsen
dc.subject.meshBiofilmsen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshStaphylococcus aureusen
dc.subject.meshSiderophoresen
dc.subject.meshBiological Productsen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshMarine Biologyen
dc.subject.meshMolecular Structureen
dc.subject.meshMicrobiotaen
dc.titleDiscovery of Biofilm Inhibitors from the Microbiota of Marine Egg Massesen
dc.title.serialJournal of Natural Productsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Scienceen
pubs.organisational-groupVirginia Tech/Science/Chemistryen
pubs.organisational-groupVirginia Tech/Faculty of Health Sciencesen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Science/COS T&R Facultyen

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