Exploring Rain as Source of Biological Control Agents for Fire Blight on Apple

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dc.contributor.authorLlontop, Marco E. Mechanen
dc.contributor.authorHurley, Kellyen
dc.contributor.authorTian, Longen
dc.contributor.authorGaleano, Vivian A. Bernalen
dc.contributor.authorWildschutte, Hans K.en
dc.contributor.authorMarine, Sasha C.en
dc.contributor.authorYoder, Keith S.en
dc.contributor.authorVinatzer, Boris A.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.contributor.departmentVirginia Agricultural Experiment Stationen
dc.date.accessioned2020-04-06T14:26:43Zen
dc.date.available2020-04-06T14:26:43Zen
dc.date.issued2020-02-14en
dc.date.updated2020-04-06T14:26:40Zen
dc.description.abstractPoor survival on plants can limit the efficacy of Biological Control Agents (BCAs) in the field. Yet bacteria survive in the atmosphere, despite their exposure to high solar radiation and extreme temperatures. If conditions in the atmosphere are similar to, or more extreme than, the environmental conditions on the plant surface, then precipitation may serve as a reservoir of robust BCAs. To test this hypothesis, two hundred and fifty-four rain-borne isolates were screened for in vitro inhibition of Erwinia amylovora, the causal agent of fire blight, as well as of other plant pathogenic bacteria, fungi and oomycetes. Two isolates showed strong activity against E. amylovora and other plant pathogenic bacteria, while other isolates showed activity against fungal and oomycete pathogens. Survival assays suggested that the two isolates that inhibited E. amylovora were able to survive on apple blossoms and branches similarly to E. amylovora. Pathogen population size and associated fire blight symptoms were significantly reduced when detached apple blossoms were treated with the two isolates before pathogen inoculation, however, disease reduction on attached blossoms within an orchard was inconsistent. Using whole genome sequencing, the isolates were identified as Pantoea agglomerans and P. ananatis, respectively. A UV-mutagenesis screen pointed to a phenazine antibiotic D-alanylgriseoluteic acid synthesis gene cluster as being at the base of the antimicrobial activity of the P. agglomerans isolate. Our work reveals the potential of precipitation as an under-explored source of BCAs, whole genome sequencing as an effective approach to precisely identify BCAs, and UV-mutagenesis as a technically simple screen to investigate the genetic basis of BCAs. More field trials are needed to determine the efficacy of the identified BCAs in fire blight control.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2020.00199en
dc.identifier.orcidVinatzer, Boris [0000-0003-4612-225X]en
dc.identifier.orcidMarine, Sasha [0000-0001-9454-1972]en
dc.identifier.urihttp://hdl.handle.net/10919/97532en
dc.language.isoenen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000517551400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectMicrobiologyen
dc.subjectrainen
dc.subjectfire blighten
dc.subjectbiocontrolen
dc.subjectgenome-based identificationen
dc.subjectUV mutagenesisen
dc.subjectRESISTANT ERWINIA-AMYLOVORAen
dc.subjectPSEUDOMONAS-FLUORESCENSen
dc.subjectPANTOEA-AGGLOMERANSen
dc.subjectBACTERIAL ANTAGONISTSen
dc.subjectSYSTEMIC RESISTANCEen
dc.subjectICE NUCLEATIONen
dc.subjectPEARen
dc.subjectSTREPTOMYCINen
dc.subjectCOLONIZATIONen
dc.subjectBLOSSOMSen
dc.subject0605 Microbiologyen
dc.titleExploring Rain as Source of Biological Control Agents for Fire Blight on Appleen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen

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