Epizootic pneumonia of bighorn sheep following experimental exposure to Mycoplasma ovipneumoniae

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dc.contributor.authorBesser, Thomas E.en
dc.contributor.authorCassirer, E. Francesen
dc.contributor.authorPotter, E. Francesen
dc.contributor.authorLahmers, Kevin K.en
dc.contributor.authorOaks, J. Lindsayen
dc.contributor.authorShanthalingam, Sudarvilien
dc.contributor.authorSrikumaran, Subramaniamen
dc.contributor.authorForeyt, William J.en
dc.date.accessioned2017-01-08T15:59:59Zen
dc.date.available2017-01-08T15:59:59Zen
dc.date.issued2014en
dc.description.abstractBACKGROUND: Bronchopneumonia is a population limiting disease of bighorn sheep (Ovis canadensis). The cause of this disease has been a subject of debate. Leukotoxin expressing Mannheimia haemolytica and Bibersteinia trehalosi produce acute pneumonia after experimental challenge but are infrequently isolated from animals in natural outbreaks. Mycoplasma ovipneumoniae, epidemiologically implicated in naturally occurring outbreaks, has received little experimental evaluation as a primary agent of bighorn sheep pneumonia. METHODOLOGY/PRINCIPAL FINDINGS: In two experiments, bighorn sheep housed in multiple pens 7.6 to 12 m apart were exposed to M. ovipneumoniae by introduction of a single infected or challenged animal to a single pen. Respiratory disease was monitored by observation of clinical signs and confirmed by necropsy. Bacterial involvement in the pneumonic lungs was evaluated by conventional aerobic bacteriology and by culture-independent methods. In both experiments the challenge strain of M. ovipneumoniae was transmitted to all animals both within and between pens and all infected bighorn sheep developed bronchopneumonia. In six bighorn sheep in which the disease was allowed to run its course, three died with bronchopneumonia 34, 65, and 109 days after M. ovipneumoniae introduction. Diverse bacterial populations, predominantly including multiple obligate anaerobic species, were present in pneumonic lung tissues at necropsy. CONCLUSIONS/SIGNIFICANCE: Exposure to a single M. ovipneumoniae infected animal resulted in transmission of infection to all bighorn sheep both within the pen and in adjacent pens, and all infected sheep developed bronchopneumonia. The epidemiologic, pathologic and microbiologic findings in these experimental animals resembled those seen in naturally occurring pneumonia outbreaks in free ranging bighorn sheep.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0110039en
dc.identifier.eissn1932-6203en
dc.identifier.issue10en
dc.identifier.urihttp://hdl.handle.net/10919/74011en
dc.identifier.volume9en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/25302992en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAnimalsen
dc.subjectLungen
dc.subjectMycoplasma ovipneumoniaeen
dc.subjectPneumoniaen
dc.subjectRNA, Ribosomal, 16Sen
dc.subjectSheepen
dc.subjectSheep Diseasesen
dc.subjectSheep, Bighornen
dc.titleEpizootic pneumonia of bighorn sheep following experimental exposure to Mycoplasma ovipneumoniaeen
dc.title.serialPLoS Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherResearch Support, Non-U.S. Gov'ten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen

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