dc.contributor.author	Vaidya, Prasad Avdhut	en
dc.contributor.committeechair	Goldstein, Aaron S.	en
dc.contributor.committeemember	Davis, Richey M.	en
dc.contributor.committeemember	Whittington, Abby R.	en
dc.contributor.department	Chemical Engineering	en
dc.date.accessioned	2015-05-23T08:09:47Z	en
dc.date.available	2015-05-23T08:09:47Z	en
dc.date.issued	2014-10-15	en
dc.description.abstract	Rupture of the anterior cruciate ligament (ACL) is one of the most common ligamentous injuries of the knee. Post rupture, the ACL does not heal on itself due to poor vasculature and hence surgical intervention is required to treat the ACL. Current surgical management of ACL rupture consists of reconstruction with autografts or allografts. However, the limitations associated with these grafts have prompted interest in tissue engineered solutions that combine cells, scaffolds and stimuli to facilitate ACL regeneration. This thesis describes a ligament tissue engineering strategy that involves incorporating biomolecules within fibers-based electrospun meshes which mimics the extra-cellular matrix microarchitecture of ligament. However, challenges exist with incorporation of biomolecules. Therefore, the goal of this research project was to develop two techniques to incorporate biomolecules within electrospun meshes: (1) co-axially electrospinning fibers that support surface-grafting of biomolecules, and (2) co-axially electrospinning fibers decorated with biomolecule-loaded microspheres. In the first approach, chitosan was co-axially electrospun on the shell side of poly caprolactone (PCL) and arginine-glycine-aspartate (RGD) was attached to the electrospun meshes. Bone marrow stromal cells (BMSCs) attached, spread and proliferated on these meshes. In the second approach, fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) loaded chitosan-alginate (CS-AL) microspheres were fabricated. The effects of cation to alginate ratio, type of alginate and concentration of CaCl2 on microsphere size, FITC-BSA loading and release were systematically evaluated. The CS-AL microspheres were then incorporated into the sheath phase of co-axially electrospun meshes to achieve microsphere-decorated fiber composite meshes. The results from these model study suggest that both approaches are tractable for incorporating biomolecules within fibers-based electrospun meshes. Both these approaches provide platform for future studies that can focus on ligament-relevant biomolecules such as FGF-2 and GDF-5.	en
dc.description.degree	Master of Science	en
dc.format.medium	ETD	en
dc.identifier.other	vt_gsexam:3749	en
dc.identifier.uri	http://hdl.handle.net/10919/52583	en
dc.publisher	Virginia Tech	en
dc.rights	In Copyright	en
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/	en
dc.subject	co-axial electrospinning	en
dc.subject	chitosan	en
dc.subject	chitosan-alginate microspheres	en
dc.subject	RGD	en
dc.subject	FITC-BSA	en
dc.subject	bone marrow stromal cells	en
dc.title	Alternative strategies to incorporate biomolecules within electrospun meshes for tissue enginering	en
dc.type	Thesis	en
thesis.degree.discipline	Chemical Engineering	en
thesis.degree.grantor	Virginia Polytechnic Institute and State University	en
thesis.degree.level	masters	en
thesis.degree.name	Master of Science	en

Alternative strategies to incorporate biomolecules within electrospun meshes for tissue enginering

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