Insights into the Preservation of the Homomorphic Sex-Determining Chromosome of Aedes aegypti from the Discovery of a Male-Biased Gene Tightly Linked to the M-Locus

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dc.contributor.authorHall, Andrew Brantleyen
dc.contributor.authorTimoshevskiy, Vladimir A.en
dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorJiang, Xiaofangen
dc.contributor.authorBasu, Sanjayen
dc.contributor.authorAnderson, Michelle A. E.en
dc.contributor.authorHu, Wanqien
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorAdelman, Zach N.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.departmentBiochemistryen
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2017-01-05T23:43:18Zen
dc.date.available2017-01-05T23:43:18Zen
dc.date.issued2014-01-01en
dc.description.abstractThe preservation of a homomorphic sex-determining chromosome in some organisms without transformation into a heteromorphic sex chromosome is a long-standing enigma in evolutionary biology. A dominant sex-determining locus (or M-locus) in an undifferentiated homomorphic chromosome confers the male phenotype in the yellow fever mosquito Aedes aegypti. Genetic evidence suggests that the M-locus is in a nonrecombining region. However, the molecular nature of the M-locus has not been characterized. Using a recently developed approach based on Illumina sequencing of male and female genomic DNA, we identified a novel gene, myo-sex, that is present almost exclusively in the male genome but can sporadically be found in the female genome due to recombination. For simplicity, we define sequences that are primarily found in the male genome as male-biased. Fluorescence in situ hybridization (FISH) on A. aegypti chromosomes demonstrated that the myo-sex probe localized to region 1q21, the established location of theM-locus.Myo-sex is a duplicated myosin heavy chain gene that is highly expressed in the pupa and adult male.Myo-sex shares 83% nucleotide identity and 97% amino acid identity with its closest autosomal paralog, consistent with ancient duplication followed by strong purifying selection. Compared with males, myo-sex is expressed at very low levels in the females that acquired it, indicating that myo-sexmay be sexually antagonistic. This study establishes a framework to discover male-biased sequences within a homomorphic sex-determining chromosome and offers new insights into the evolutionary forces that have impeded the expansion of the nonrecombining M-locus in A. aegypti.en
dc.description.versionPublished versionen
dc.format.extent179 - 191 (13) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/gbe/evu002en
dc.identifier.issn1759-6653en
dc.identifier.issue1en
dc.identifier.urihttp://hdl.handle.net/10919/73979en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000331289100015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 3.0 Unporteden
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectEvolutionary Biologyen
dc.subjectGenetics & Heredityen
dc.subjectsex-determinationen
dc.subjectsex chromosomesen
dc.subjectchromosome quotienten
dc.subjectmyo-sexen
dc.subjectnonrecombining regionen
dc.subjectsexual antagonismen
dc.subjectYELLOW-FEVER MOSQUITOen
dc.subjectSILK FIBROIN GENEen
dc.subjectY-CHROMOSOMEen
dc.subjectLINKAGE MAPen
dc.subjectCULEX-PIPIENSen
dc.subjectHUMAN GENOMEen
dc.subjectEVOLUTIONen
dc.subjectDROSOPHILAen
dc.subjectANOPHELESen
dc.subjectSEQUENCEen
dc.titleInsights into the Preservation of the Homomorphic Sex-Determining Chromosome of Aedes aegypti from the Discovery of a Male-Biased Gene Tightly Linked to the M-Locusen
dc.title.serialGenome Biology And Evolutionen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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