Antibacterial activity and safety of commercial veterinary cationic steroid antibiotics and neutral superoxidized water

Simple item page
dc.contributor.authorBergstrom, Benjamin E.en
dc.contributor.authorAbdelKhalek, Ahmeden
dc.contributor.authorYounis, Waleeden
dc.contributor.authorHammac, G. Kenitraen
dc.contributor.authorTownsend, Wendy M.en
dc.contributor.authorSeleem, Mohamed N.en
dc.date.accessioned2020-09-21T16:09:10Zen
dc.date.available2020-09-21T16:09:10Zen
dc.date.issued2018-03-07en
dc.date.updated2020-09-21T16:09:06Zen
dc.description.abstractAntibiotic resistance of bacteria common to the ocular surface is an evolving problem. Thus, novel treatment options with new modes of action are required. We investigated the antibacterial activity and safety of three commercially available topical veterinary ophthalmic products (cationic steroid antibiotics, products A and B, and a neutral superoxidized water, product C) to determine their potential use as antimicrobial alternatives. The minimum inhibitory concentrations (MIC) of the three products were determined against 17 antibiotic resistant bacterial clinical isolates from the ocular surface. Using a standard cytotoxicity assay, the products at varying concentrations were evaluated with a corneal fibroblast cell line and a macrophage-like cell line to determine their potential toxic effect in vitro. The commercial ophthalmic solutions, ofloxacin 0.3%, tobramycin 0.3% and gentamicin 0.3% were used as positive controls for the MIC and tobramycin 0.3% was used as positive control for the cytotoxicity assays. For the MIC, Product C showed no inhibition of growth for any organisms, while Products A and B showed inhibition of growth similar to slightly less than the positive controls. For the cytotoxicity assays, Product C exhibited minimal toxicity while Products A and B exhibited toxicity similar to the controls. In conclusion, Product C had no antibacterial activity in these assays, while Products A and B had antibacterial profiles similar to slightly less than common topical ophthalmic antibiotics and cytotoxicity profiles similar to common topical ophthalmic antibiotics. To our knowledge, this is the first report on the antibacterial activity and safety of the cationic steroid antibiotics and superoxidized water.en
dc.description.versionPublished versionen
dc.format.extent13 page(s)en
dc.format.mediumElectronic-eCollectionen
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e0193217 (Article number)en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0193217en
dc.identifier.eissn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.issue3en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.otherPONE-D-17-25690 (PII)en
dc.identifier.pmid29513686 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/100016en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherPLoSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMETHICILLIN-RESISTANT STAPHYLOCOCCUSen
dc.subjectACID AQUEOUS-SOLUTIONen
dc.subjectANTIMICROBIAL PEPTIDESen
dc.subjectPOLYMYXIN-Ben
dc.subjectPSEUDOMONAS-AERUGINOSAen
dc.subjectCERAGENINSen
dc.subjectPHen
dc.subjectINFECTIONSen
dc.subjectPREVALENCEen
dc.subjectEFFICACYen
dc.subject.meshEyeen
dc.subject.meshCell Lineen
dc.subject.meshAnimalsen
dc.subject.meshDogsen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshBacteriaen
dc.subject.meshEye Infections, Bacterialen
dc.subject.meshDog Diseasesen
dc.subject.meshHydrogen Peroxideen
dc.subject.meshCationsen
dc.subject.meshOfloxacinen
dc.subject.meshSteroidsen
dc.subject.meshGentamicinsen
dc.subject.meshTobramycinen
dc.subject.meshOphthalmic Solutionsen
dc.subject.meshVeterinary Drugsen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshDrug Resistance, Microbialen
dc.titleAntibacterial activity and safety of commercial veterinary cationic steroid antibiotics and neutral superoxidized wateren
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2018-01-27en
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Techen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Antibacterial activity and safety of commercial veterinary cationic steroid antibiotics and neutral superoxidized water.pdf
Size:
1.61 MB
Format:
Adobe Portable Document Format
Download

Collections

All Faculty Deposits
Scholarly Works, Biomedical Sciences and Pathobiology