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Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China

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dc.contributor.authorTang, Yien
dc.contributor.authorZhao, Kaien
dc.contributor.authorYin, Hong-Minen
dc.contributor.authorYang, Li-Pingen
dc.contributor.authorWu, Yue-Chunen
dc.contributor.authorLi, Feng-Yien
dc.contributor.authorYang, Zeen
dc.contributor.authorLu, Hui-Xuanen
dc.contributor.authorWang, Boen
dc.contributor.authorYang, Yinen
dc.contributor.authorZhang, Yun-Zhien
dc.contributor.authorYang, Xing-Louen
dc.date.accessioned2024-06-26T13:34:03Zen
dc.date.available2024-06-26T13:34:03Zen
dc.date.issued2024-06-17en
dc.date.updated2024-06-26T13:23:10Zen
dc.description.abstractHepatitis A virus (HAV), a member of the genus <i>Hepatovirus</i> (<i>Picornaviridae</i> HepV), remains a significant viral pathogen, frequently causing enterically transmitted hepatitis worldwide. In this study, we conducted an epidemiological survey of HepVs carried by small terrestrial mammals in the wild in Yunnan Province, China. Utilizing HepV-specific broad-spectrum RT-PCR, next-generation sequencing (NGS), and QNome nanopore sequencing (QNS) techniques, we identified and characterized two novel HepVs provisionally named EpMa-HAV and EpLe-HAV, discovered in the long-tailed mountain shrew (<i>Episoriculus macrurus</i>) and long-tailed brown-toothed shrew (<i>Episoriculus leucops</i>), respectively. Our sequence and phylogenetic analyses of EpMa-HAV and EpLe-HAV indicated that they belong to the species <i>Hepatovirus I</i> (HepV-I) clade II, also known as the Chinese shrew HepV clade. Notably, the codon usage bias pattern of novel shrew HepVs is consistent with that of previously identified Chinese shrew HepV. Furthermore, our structural analysis demonstrated that shrew HepVs differ from other mammalian HepVs in RNA secondary structure and exhibit variances in key protein sites. Overall, the discovery of two novel HepVs in shrews expands the host range of HepV and underscores the existence of genetically diverse animal homologs of human HAV within the genus HepV.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationTang, Y.; Zhao, K.; Yin, H.-M.; Yang, L.-P.; Wu, Y.-C.; Li, F.-Y.; Yang, Z.; Lu, H.-X.; Wang, B.; Yang, Y.; Zhang, Y.-Z.; Yang, X.-L. Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China. Viruses 2024, 16, 969.en
dc.identifier.doihttps://doi.org/10.3390/v16060969en
dc.identifier.urihttps://hdl.handle.net/10919/119516en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecthepatitis A virus (HAV)en
dc.subjectHepatovirus (HepV)en
dc.subjectshrewen
dc.subjectnext-generation sequencing (NGS)en
dc.subjectamplicon and QNome nanopore sequencing (A-QNS)en
dc.subjectcodon usage biasen
dc.subjectRNA secondary structureen
dc.subjectclassificationen
dc.subjectevolutionen
dc.titleIdentification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, Chinaen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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